Hontzeas N, Richardson A O, Belimov A, Safronova V, Abu-Omar M M, Glick B R
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Appl Environ Microbiol. 2005 Nov;71(11):7556-8. doi: 10.1128/AEM.71.11.7556-7558.2005.
PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical transmission. The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT.
聚合酶链反应（PCR）被用于从细菌中快速鉴定和分离1-氨基环丙烷-1-羧酸（ACC）脱氨酶基因。使用Shimodaira-Hasegawa检验来评估暗示水平基因转移（HGT）的系统发育异常基因定位是否比垂直传播更受显著青睐。最佳的最大似然（ML）ACC脱氨酶树比四个替代的ML树具有显著更高的可能性，表明存在水平基因转移。
