Fu Bo, Wang Weiwei, Tang Ming, Chen Xingdu
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China.
Wei Sheng Wu Xue Bao. 2009 Mar;49(3):395-9.
We used Medicago sativa rhizosphere in Shaanxi province of China to isolate and identify hydrogen-oxidizing bacteria that produced ACC (1-aminocyclopropane-1-carboxylate) deaminase, and then studied the mechanism why they can promote the growth of plants.
Hydrogen-oxidizing bacteria were isolated by gas-cycle incubation system. We studied the morphological character, physiological characteristics, 16S rDNA sequence analysis and built the phylogenic tree. Thin layer chromatography was used to isolate the strain that produced ACC deaminase. Ninhydrin reaction was used to test the enzyme activity.
In total 37 strains were isolated, 8 of which could oxidize H2 strongly and grow chemolithoautotrophically. We initially identified them as hydrogen-oxidizing bacteria. Only strain WMQ-7 produced ACC deaminase among these 8 strains. Morphological and physiological characteristics analysis showed that strain WMQ-7 was essentially consistent with Pseudomonas putida. The 16S rDNA sequence analysis (GenBank accession number EU807744) suggested that strain WMQ-7 was clustered together with Pseudomonas putida in phylogenetic tree, with the sequence identity of 99%. Based on all these results, strain WMQ-7 was identified as Pseudomonas putida. The enzyme activity of strain WMQ-7 was 0.671 U/microg.
A strain producing ACC deaminase was identified and tested.
利用中国陕西省紫花苜蓿根际土壤分离鉴定产1-氨基环丙烷-1-羧酸（ACC）脱氨酶的氢氧化细菌，并研究其促进植物生长的机制。
采用气体循环培养系统分离氢氧化细菌。研究其形态特征、生理特性、16S rDNA序列分析并构建系统发育树。用薄层层析法分离产ACC脱氨酶的菌株，用茚三酮反应检测酶活性。
共分离出37株菌，其中8株能强烈氧化H2并进行化能无机自养生长，初步鉴定为氢氧化细菌。这8株菌中只有WMQ-7菌株产ACC脱氨酶。形态和生理特性分析表明，WMQ-7菌株与恶臭假单胞菌基本一致。16S rDNA序列分析（GenBank登录号EU807744）显示，WMQ-7菌株在系统发育树中与恶臭假单胞菌聚在一起，序列同源性为99%。综合各项结果，WMQ-7菌株被鉴定为恶臭假单胞菌。WMQ-7菌株的酶活性为0.671 U/μg。
鉴定并检测了1株产ACC脱氨酶的菌株。
