Stuart A J, Smith D A
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Avian Dis. 1992 Apr-Jun;36(2):447-9.
The fluorescent calcium binding dyes xylenol orange, calcein green, and tetracycline were used successfully to sequentially label new bone deposition and bone remodeling during the healing of rigidly fixed humeral fractures in the chicken. Xylenol orange and calcein green, administered subcutaneously at 90 mg/kg and 10 mg/kg, respectively, provided easily identified and differentiated bands in newly deposited bone within periosteal and endosteal calluses, intercortical gaps, and screw holes. Tetracycline, administered at 50 mg/kg per os by crop tube, labeled bone faintly in a non-specific manner. Parenteral administration of the same compound might have yielded better results.
荧光钙结合染料二甲酚橙、钙黄绿素和四环素成功用于依次标记鸡肱骨骨折坚强内固定愈合过程中的新骨沉积和骨重塑。分别以90mg/kg和10mg/kg的剂量皮下注射二甲酚橙和钙黄绿素，在骨膜和骨内膜骨痂、皮质间间隙和螺孔内的新沉积骨中产生易于识别和区分的条带。通过嗉囊管以50mg/kg的剂量经口给予四环素，以非特异性方式对骨进行微弱标记。肠胃外给予相同化合物可能会产生更好的结果。
