Ohuchi Yoshie, Myojin Yuki, Shimamoto Fumio, Kashimoto Naoki, Kamiya Kenji, Watanabe Hiromitsu
Department of Health Science, Hiroshima Prefectural Women's University, 1-1-71 Ujina-higashi, Minami-ku, Hiroshima 734-8558, Japan.
Oncol Rep. 2005 Dec;14(6):1559-64.
The present study was designed to investigate the effects of fermented miso (fermented soybean paste) on the induction of colon tumors by azoxymethane (AOM) in male F344 rats. A total of 91 rats, 6 weeks of age, were divided into 5 groups and given weekly subcutaneous injections of AOM (15 mg/kg body wt) for 3 weeks. The animals were placed on diets one week before the first AOM dose: commercial normal control MF diet or a diet containing 10% 2-year, 180-day fermented, or 3-4-day fermented miso. There were no differences in body and organ weights, and no aberrant crypt foci (ACF) among carcinogen-treated groups at week 25. The rates of tumor incidence were 45%, 85%, 75% and 60% with the 2-year, 180-day, and 3-4-day fermented miso and MF, respectively, and those for colon tumors were 34%, 55%, 60% and 55%, respectively. The size of well-differentiated adenocarcinomas and total (well differentiated and signet ring cell) adenocarcinomas in the 180-day fermented miso group was significantly smaller than that in the 2-year fermented miso and MF+AOM groups. Nuclear staining of beta-catenin in colon tumors was increased for the 3-4-day fermented miso compared to the 180-day fermented miso. Cdx2 staining tendency was decreased in colon tumors and adenocarcinomas compared to normal mucosa and ACF, which stained in 100% of cases. In addition, the PCNA index was significantly reduced in the 180-day group compared with those groups receiving the 3-4-day fermented miso and MF diet. The germinal region was also decreased. The present results indicate that dietary supplementation with 180-day fermented dietary miso could act as a chemopreventive agent for colon carcinogenesis.
本研究旨在调查发酵味噌（发酵大豆酱）对雄性F344大鼠中由偶氮甲烷（AOM）诱导的结肠肿瘤的影响。总共91只6周龄的大鼠被分为5组，并每周皮下注射AOM（15 mg/kg体重），持续3周。在首次注射AOM前一周，将动物置于不同饮食中：市售正常对照MF饮食或含10%两年期、180天发酵或3 - 4天发酵味噌的饮食。在第25周时，致癌物处理组之间的体重和器官重量以及异常隐窝灶（ACF）没有差异。两年期、180天、3 - 4天发酵味噌和MF组的肿瘤发生率分别为45%、85%、75%和60%，结肠肿瘤的发生率分别为34%、55%、60%和55%。180天发酵味噌组中高分化腺癌和总（高分化和印戒细胞）腺癌的大小明显小于两年期发酵味噌和MF + AOM组。与180天发酵味噌相比，3 - 4天发酵味噌的结肠肿瘤中β - 连环蛋白的核染色增加。与正常黏膜和ACF（100%病例染色）相比，结肠肿瘤和腺癌中的Cdx2染色趋势降低。此外，与接受3 - 4天发酵味噌和MF饮食的组相比，180天组的增殖细胞核抗原（PCNA）指数显著降低。生发区也减少。目前的结果表明，补充180天发酵的饮食味噌可作为结肠癌变的化学预防剂。
