Ohara Masayuki, Lu Huimei, Shiraki Katsutomo, Ishimura Yoshiyuki, Uesaka Toshihiro, Katoh Osamu, Watanabe Hiromitsu
Department of Environment and Mutation, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8553, Japan.
Oncol Rep. 2002 Jan-Feb;9(1):69-73.
The present study was designed to investigate the effects of fermented miso in the diet on the induction of aberrant crypt foci (ACF) by azoxymethane (AOM) in male F344 rats. A total of 50 rats, 8 weeks of age, were divided into 5 groups and given weekly subcutaneous injections of AOM (15 mg/kg body wt) for 3 weeks. Rats were fed a normal control MF solid diet, or solid diet containing 10% long-term fermented (aged), medium- or short-term fermented miso, or 2.2% NaCl for 5 weeks, starting one week before the first AOM dosing. It was found that, compared to the control (MF) diet, the long-term fermented diet significantly decreased (by 22.2%) ACF/colon, but increased (by 18.2%) the number of aberrant crypts (Acs)/focus. The latter was also increased by the medium-term fermented diet (by 25.3%). The PCNA labeling index was only affected by the short-term fermented diet (36.9% increase) and by 2.2% NaCl diet (27.2% increased). The present results indicate that aged or completely fermented miso supplemented into the diet, could act as a chemopreventive agent for colon carcinogenesis.
本研究旨在调查饮食中发酵味噌对雄性F344大鼠中由氧化偶氮甲烷（AOM）诱导的异常隐窝灶（ACF）的影响。总共50只8周龄的大鼠被分为5组，并连续3周每周皮下注射AOM（15毫克/千克体重）。从首次给予AOM前一周开始，大鼠被喂食正常对照MF固体饮食，或含有10%长期发酵（陈酿）、中期或短期发酵味噌的固体饮食，或2.2%氯化钠，持续5周。结果发现，与对照（MF）饮食相比，长期发酵饮食显著降低了ACF/结肠（降低了22.2%），但增加了每个病灶中异常隐窝（Ac）的数量（增加了18.2%）。中期发酵饮食也增加了后者（增加了25.3%）。增殖细胞核抗原（PCNA）标记指数仅受短期发酵饮食（增加36.9%）和2.2%氯化钠饮食（增加27.2%）的影响。目前的结果表明，饮食中添加陈酿或完全发酵的味噌可作为结肠癌发生的化学预防剂。
