Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain.
Methods Mol Biol. 2021;2271:47-56. doi: 10.1007/978-1-0716-1241-5_3.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be regarded as a key tool to rapidly obtain molecular mass information of intact glycoproteins in glycoproteomic studies and quality control of recombinant biopharmaceuticals. However, MALDI-TOF MS of these glycosylated compounds is a tricky task due to its low ionization efficiency and fragmentation of labile groups such as sialic acids.Here, we offer the reader a practical overview of the available methodologies for the confident analysis of intact glycoproteins with different glycosylation degree by MALDI-TOF MS. The three proposed methods fulfil the requirements of reproducibility and low extent of glycan fragmentation required to successfully analyze intact glycoproteins.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）可被视为一种快速获取糖蛋白组学研究中完整糖蛋白的分子量信息和重组生物制药质量控制的关键工具。然而，由于其低离子化效率和唾液酸等不稳定基团的碎片化，MALDI-TOF MS 分析这些糖基化化合物是一项棘手的任务。在这里，我们为读者提供了一个实用的概述，介绍了通过 MALDI-TOF MS 分析具有不同糖基化程度的完整糖蛋白的可用方法。这三种方法都满足了重现性和低聚糖碎片化程度的要求，这是成功分析完整糖蛋白所必需的。
