Xia Ying, Min Hang
College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Wei Sheng Wu Xue Bao. 2003 Dec;43(6):691-7.
A bacterium (designated strain ZX4) able to degrade poly aromatic hydrocarbons was isolated from oil-contaminated soil. Based on analysis of the 16S rDNA sequence, whole cell fatty acid, and Biolog-GN, the strain was identified as Sphingomonas paucimobilis. The detection of GST (Glutathione S-transferase) indicated that the GST from the strain had the activity of conjugating with CDNB and it maybe relate with degradation of PAHs. The PCR product of GST gene from the strain confirmed its presence. Phylogeny analysis based on gst sequence indicated that the strain was relatively close with the strain which can degrade PAH likewise.
从石油污染土壤中分离出一株能够降解多环芳烃的细菌（命名为ZX4菌株）。基于16S rDNA序列、全细胞脂肪酸分析以及Biolog-GN分析，该菌株被鉴定为少动鞘氨醇单胞菌。谷胱甘肽S-转移酶（GST）的检测表明，该菌株的GST具有与1-氯-2,4-二硝基苯（CDNB）结合的活性，这可能与多环芳烃的降解有关。该菌株GST基因的PCR产物证实了其存在。基于gst序列的系统发育分析表明，该菌株与同样能够降解多环芳烃的菌株亲缘关系相对较近。
