Baron T, Doré J F
INSERM Unit 218, Centre Léon Bérard, Lyon, France.
In Vivo. 1992 Jan-Feb;6(1):77-9.
Canine lymphoid tumours, which share a number of features with human non-Hodgkin's lymphomas, were grafted in nude or SCID mice. Two (DL.24,DL.31) out of eight lymphomas and two (DL.31,DL.35) out of three lymphomas produced a sub-cutaneous (s.c.) tumour in nude and SCID mice respectively. In all animals, the s.c. tumours that developed at the inoculation site were regularly associated with metastasis to the regional lymph nodes, and also to the spleen, liver and bone marrow in SCID mice. The four transplanted tumours, with a pseudo-diploid canine karyotype, were diffuse large cell lymphomas as the initial dog tumours, and could be immunophenotypically characterized by surface immunoglobulins, MHC-class 2 and Thy-1 antigens. Serially transplanted lymphomas in nude and SCID mice may hence be used for further studies of these tumours.
与人类非霍奇金淋巴瘤有许多共同特征的犬类淋巴瘤被移植到裸鼠或严重联合免疫缺陷（SCID）小鼠体内。8个淋巴瘤中有2个（DL.24、DL.31）和3个淋巴瘤中有2个（DL.31、DL.35）分别在裸鼠和SCID小鼠体内产生了皮下肿瘤。在所有动物中，接种部位形成的皮下肿瘤经常伴有区域淋巴结转移，在SCID小鼠中还伴有脾脏、肝脏和骨髓转移。这4个移植肿瘤具有假二倍体犬类核型，最初的犬类肿瘤为弥漫性大细胞淋巴瘤，可通过表面免疫球蛋白、MHC-2类和Thy-1抗原进行免疫表型特征分析。因此，在裸鼠和SCID小鼠中连续移植的淋巴瘤可用于这些肿瘤的进一步研究。
