Baron T, Rigal D, Bryon P A, Bonnard M, Grapin R, Doré J F
Inserm U 218, Centre Léon Bérard, France.
Anticancer Res. 1991 Sep-Oct;11(5):1751-4.
A continuous canine lymphoma cell line (DL. 24N) was established by serial transplantation from a diffuse large cell lymphoma into athymic nude mice, yielding subcutaneous tumours at the injection site associated with lymph node metastasis. The transplanted tumour cells, of canine origin as assessed by the karyotype, appear comparable to the large non-cleaved and immunoblastic human cells, as did the initial tumour cells. Immunological studies show reactivity with antibodies to dog immunoglobulins, DT-2 and Thy-1, to MHC-class II antigens, and to both CD10 (CALLA) and CD21 (CR2) human antigens. Such a cell line, showing similarities with some of the human diffuse large cell lymphomas, will thus provide a new tool for further comparative studies of malignant lymphomas.
通过将弥漫性大细胞淋巴瘤连续移植到无胸腺裸鼠体内，建立了一种犬淋巴瘤细胞系（DL. 24N），在注射部位产生皮下肿瘤并伴有淋巴结转移。通过核型分析评估，移植的肿瘤细胞来源于犬，与最初的肿瘤细胞一样，类似于大的无裂和免疫母细胞性人类细胞。免疫学研究表明，该细胞系与抗犬免疫球蛋白、DT-2和Thy-1抗体、MHC-II类抗原以及CD10（CALLA）和CD21（CR2）人类抗原均有反应。这样一种与某些人类弥漫性大细胞淋巴瘤具有相似性的细胞系，将为恶性淋巴瘤的进一步比较研究提供新的工具。
