Chang Chih-Hung, Kuo Tzong-Fu, Lin Chien-Cheng, Chou Cheng-Hung, Chen Kuang-Ho, Lin Feng-Huei, Liu Hwa-Chang
Institute of Biomedical Engineering, National Taiwan University, 7 Chung-Shan South Road, Taipei 100, Taiwan, ROC.
Biomaterials. 2006 Mar;27(9):1876-88. doi: 10.1016/j.biomaterials.2005.10.014. Epub 2005 Nov 8.
We previously showed that cartilage tissue can be engineered in vitro with porcine chondrocytes and gelatin/chondoitin-6-sulfate/hyaluronan tri-copolymer which mimic natural cartilage matrix for use as a scaffold. In this animal study, 15 miniature pigs were used in a randomized control study to compare tissue engineering with allogenous chondrocytes, autogenous osteochondral (OC) transplantation, and spontaneous repair for OC articular defects. In another study, 6 pigs were used as external controls in which full thickness (FT) and OC defects were either allowed to heal spontaneously or were filled with scaffold alone. After exclusion of cases with infection and secondary arthritis, the best results were obtained with autogenous OC transplantation, except that integration into host cartilage was poor. The results for the tissue engineering-treated group were satisfactory, the repair tissue being hyaline cartilage and/or fibrocartilage. Spontaneous healing and filling with scaffold alone did not result in good repair. With OC defects, the subchondral bone plate was not restored by cartilage tissue engineering. These results show that tri-copolymer can be used in in vivo cartilage tissue engineering for the treatment of FT articular defects.
我们之前表明，猪软骨细胞与明胶/硫酸软骨素-6/透明质酸三元共聚物可在体外构建软骨组织，该三元共聚物可模拟天然软骨基质用作支架。在这项动物研究中，15只小型猪被用于一项随机对照研究，以比较同种异体软骨细胞组织工程、自体骨软骨（OC）移植和OC关节缺损的自然修复效果。在另一项研究中，6只猪用作外部对照，其全层（FT）和OC缺损要么任其自然愈合，要么仅用支架填充。排除感染和继发性关节炎病例后，自体OC移植取得了最佳效果，只是与宿主软骨的整合较差。组织工程治疗组的结果令人满意，修复组织为透明软骨和/或纤维软骨。自然愈合和仅用支架填充均未实现良好修复。对于OC缺损，软骨组织工程未使软骨下骨板得到修复。这些结果表明，三元共聚物可用于体内软骨组织工程以治疗FT关节缺损。
