Peck Yvonne, He Pengfei, Chilla Geetha Soujanya V N, Poh Chueh Loo, Wang Dong-An
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore.
Sci Rep. 2015 Nov 9;5:16225. doi: 10.1038/srep1622510.1038/srep16225.
In this pilot study, an autologous synthetic scaffold-free construct with hyaline quality, termed living hyaline cartilaginous graft (LhCG), was applied for treating cartilage lesions. Implantation of autologous LhCG was done at load-bearing regions of the knees in skeletally mature mini-pigs for 6 months. Over the course of this study, significant radiographical improvement in LhCG treated sites was observed via magnetic resonance imaging. Furthermore, macroscopic repair was effected by LhCG at endpoint. Microscopic inspection revealed that LhCG engraftment restored cartilage thickness, promoted integration with surrounding native cartilage, produced abundant cartilage-specific matrix molecules, and re-established an intact superficial tangential zone. Importantly, the repair efficacy of LhCG was quantitatively shown to be comparable to native, unaffected cartilage in terms of biochemical composition and biomechanical properties. There were no complications related to the donor site of cartilage biopsy. Collectively, these results imply that LhCG engraftment may be a viable approach for articular cartilage repair.
在这项试点研究中，一种具有透明软骨质量的自体无合成支架构建物，称为活透明软骨移植物（LhCG），被用于治疗软骨损伤。在骨骼成熟的小型猪的膝关节承重区域植入自体LhCG，为期6个月。在本研究过程中，通过磁共振成像观察到LhCG治疗部位有显著的影像学改善。此外，在研究终点时LhCG实现了宏观修复。显微镜检查显示，LhCG植入恢复了软骨厚度，促进了与周围天然软骨的整合，产生了丰富的软骨特异性基质分子，并重新建立了完整的表面切线区。重要的是，在生化组成和生物力学特性方面，LhCG的修复效果经定量显示与天然未受影响的软骨相当。没有与软骨活检供体部位相关的并发症。总体而言，这些结果表明LhCG植入可能是一种可行的关节软骨修复方法。
