Kalyuzhnaya Marina G, Stolyar Sergey M, Auman Ann J, Lara Jimmie C, Lidstrom Mary E, Chistoserdova Ludmila
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2345-2350. doi: 10.1099/ijs.0.63405-0.
An obligately methanotrophic bacterial strain, LW14T, isolated from the sediment of Lake Washington, Seattle, USA, is described taxonomically. The isolate is an aerobic, Gram-negative, non-motile bacterium capable of growth on methane, and possesses type I intracytoplasmic membranes (i.e. it is a type I methanotroph). The strain possesses particulate methane monooxygenase (MMO) and has no soluble MMO. Formaldehyde is assimilated via the ribulose monophosphate cycle. The isolate grows within a pH range of 4-8, with the optimum between pH 5.5 and 6.5. The cellular fatty acid profile is dominated by C(16 : )omega18c, C(16 : 1)omega7c and C(16 : 1)omega5t fatty acids. The DNA G+C content is 53.3+/-0.4 mol%. On the basis of sequence analysis of the 16S rRNA gene, isolate LW14T is related most closely to representatives of the genus Methylosarcina. However, DNA-DNA hybridization analysis reveals only a distant relationship between isolate LW14T and the previously described Methylosarcina species. On the basis of its phenotypic and genotypic characteristics, LW14T represents a novel species of the genus Methylosarcina, for which the name Methylosarcina lacus sp. nov. is proposed, with LW14T (=ATCC BAA-1047T=JCM 13284T) as the type strain.
对从美国西雅图华盛顿湖沉积物中分离出的一株专性甲烷营养细菌菌株LW14T进行了分类学描述。该分离株是一种需氧、革兰氏阴性、不运动的细菌，能够利用甲烷生长，具有I型胞内膜（即它是I型甲烷营养菌）。该菌株具有颗粒性甲烷单加氧酶（MMO），没有可溶性MMO。甲醛通过磷酸戊糖途径被同化。该分离株在pH值4 - 8的范围内生长，最适pH值在5.5至6.5之间。细胞脂肪酸谱以C(16 : )ω18c、C(16 : 1)ω7c和C(16 : 1)ω5t脂肪酸为主。DNA的G + C含量为53.3±0.4 mol%。基于16S rRNA基因的序列分析，分离株LW14T与甲基八叠球菌属的代表菌株关系最为密切。然而，DNA - DNA杂交分析表明，分离株LW14T与先前描述的甲基八叠球菌属物种之间只有远缘关系。基于其表型和基因型特征，LW14T代表甲基八叠球菌属的一个新物种，为此提出新名称甲基湖八叠球菌（Methylosarcina lacus sp. nov.），以LW14T（=ATCC BAA - 1047T = JCM 13284T）作为模式菌株。
