de Groot Arjan, Chapon Virginie, Servant Pascale, Christen Richard, Saux Marion Fischer-Le, Sommer Suzanne, Heulin Thierry
Laboratoire d'Écologie Microbienne de la Rhizosphère (LEMIR), UMR 6191 CNRS-CEA-Université de la Méditerranée, DSV-DEVM, CEA Cadarache, F-13108 Saint-Paul-Lez-Durance, France.
Institut de Génétique et Microbiologie, UMR 8621 CNRS-Université Paris-Sud, LRC CEA 42V, Bâtiment 409, F-91405 Orsay cedex, France.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2441-2446. doi: 10.1099/ijs.0.63717-0.
Two gamma- and UV-radiation-tolerant, Gram-negative, rod-shaped bacterial strains, VCD115T and VCD117, were isolated from a mixture of sand samples collected in the Sahara Desert in Morocco and Tunisia, after exposure of the sand to 15 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridizations showed that VCD115T and VCD117 are members of a novel species belonging to the genus Deinococcus, with Deinococcus grandis as its closest relative. The DNA G+C contents of VCD115T and VCD117 are 59.8 and 60.6 mol%, respectively. The major fatty acids (straight-chain 15 : 1, 16 : 1, 17 : 1 and 16 : 0), polar lipids (dominated by phosphoglycolipids and glycolipids) and quinone type (MK-8) support the affiliation to the genus Deinococcus. The strains did not grow on rich medium such as trypticase soy broth (TSB), but did grow as whitish colonies on tenfold-diluted TSB. The genotypic and phenotypic properties allowed differentiation of VCD115T and VCD117 from recognized Deinococcus species. Strains VCD115T and VCD117 are therefore identified as representing a novel species, for which the name Deinococcus deserti sp. nov. is proposed, with the type strain VCD115T (=DSM 17065T=LMG 22923T).
从摩洛哥和突尼斯撒哈拉沙漠采集的混合沙样中，经15千戈瑞伽马辐射处理后，分离出两株耐伽马和紫外线辐射的革兰氏阴性杆状细菌菌株VCD115T和VCD117。基于16S rRNA基因序列的系统发育分析和DNA - DNA杂交表明，VCD115T和VCD117是属于嗜皮菌属的一个新物种的成员，与巨大嗜皮菌亲缘关系最近。VCD115T和VCD117的DNA G + C含量分别为59.8和60.6摩尔%。主要脂肪酸（直链15 : 1、16 : 1、17 : 1和16 : 0）、极性脂质（以磷酸糖脂和糖脂为主）和醌型（MK - 8）支持其归属于嗜皮菌属。这些菌株在胰蛋白酶大豆肉汤（TSB）等丰富培养基上不生长，但在稀释十倍的TSB上能形成白色菌落。其基因型和表型特性使VCD115T和VCD117能够与已确认的嗜皮菌属物种区分开来。因此，菌株VCD115T和VCD117被鉴定为代表一个新物种，提议将其命名为沙漠嗜皮菌（Deinococcus deserti sp. nov.），模式菌株为VCD115T（=DSM 17065T = LMG 22923T）。
