Zhang Yu-Qin, Sun Cheng-Hang, Li Wen-Jun, Yu Li-Yan, Zhou Jian-Qin, Zhang Yue-Qin, Xu Li-Hua, Jiang Cheng-Lin
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, People's Republic of China.
Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):370-375. doi: 10.1099/ijs.0.64292-0.
A Gram-negative, non-spore-forming, non-motile, rod-shaped, red-pigmented strain, designated YIM 007T, was found as a contaminant on an agar plate in the laboratory of Yunnan Institute of Microbiology, China. The optimum growth pH and temperature for the isolate were 7.0-7.5 and 30 degrees C, respectively. The predominant respiratory quinone was MK-8. The polar lipid profile consisted mainly of various unknown phosphoglycolipids and glycolipids. The major cellular fatty acids were C(16 : 1)omega7c, C(16 : 0), C(17 : 0) and C(17 : 1)omega8c. l-Ornithine was detected in its peptidoglycan. The DNA G+C content was 64.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 007(T) showed 16S rRNA gene sequence similarity levels of 86.8-92.1 % to the other described Deinococcus species. Based on the high 16S rRNA gene sequence divergence and phenotypic differences, it is proposed that the unknown strain should be classified as a novel species in the genus Deinococcus with the name Deinococcus yunweiensis sp. nov. The type strain is YIM 007T (=KCTC 3962T=DSM 17005T).
在中国云南微生物研究所的实验室中,在一个琼脂平板上发现了一株革兰氏阴性、不形成芽孢、无运动性、杆状、产红色色素的菌株,命名为YIM 007T。该菌株的最适生长pH值和温度分别为7.0 - 7.5和30℃。主要呼吸醌为MK - 8。极性脂质谱主要由各种未知的磷酸糖脂和糖脂组成。主要的细胞脂肪酸为C(16 : 1)ω7c、C(16 : 0)、C(17 : 0)和C(17 : 1)ω8c。在其肽聚糖中检测到L - 鸟氨酸。DNA G + C含量为64.1 mol%。基于16S rRNA基因序列的系统发育分析表明,菌株YIM 007(T)与其他已描述的嗜皮菌属物种的16S rRNA基因序列相似性水平为86.8 - 92.1%。基于高16S rRNA基因序列差异和表型差异,建议将该未知菌株归为嗜皮菌属的一个新物种,命名为云南嗜皮菌(Deinococcus yunweiensis sp. nov.)。模式菌株为YIM 007T(=KCTC 3962T=DSM 17005T)。