Lau Stanley C K, Tsoi Mandy M Y, Li Xiancui, Plakhotnikova Ioulia, Dobretsov Sergey, Wong Po-Keung, Qian Pei-Yuan
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2497-2500. doi: 10.1099/ijs.0.63824-0.
A yellow-pigmented, Gram-negative, slowly gliding, rod-shaped, strictly aerobic bacterium (UST040801-001T) was isolated from marine sediment. The DNA G+C content was 39.9 mol%. The predominant fatty acids were a15 : 0, i15 : 0, i15 : 0 3-OH, i17 : 1omega9c, i17 : 0 3-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1omega7c (altogether representing 76.2 % of the total). MK-6 was the only respiratory quinone. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences indicated that Gramella echinicola KMM 6050T (the only species in the genus) was the closest relative of UST040801-001T, sharing 98.0 % sequence similarity. The DNA-DNA relatedness between UST040801-001T and Gramella echinicola KMM 6050T was 13 %. Strain UST040801-001T can be distinguished from G. echinicola by means of 11 phenotypic traits. The results of molecular and phenotypic analyses suggested that UST040801-001T represents a novel species of Gramella. The name Gramella portivictoriae sp. nov. is proposed for this bacterium, with UST040801-001T (=NRRL B-41137T [corrected] =JCM 13192T) as the type strain.
从海洋沉积物中分离出一株黄色色素、革兰氏阴性、缓慢滑行、杆状、严格需氧的细菌（UST040801-001T）。DNA的G+C含量为39.9 mol%。主要脂肪酸为a15 : 0、i15 : 0、i15 : 0 3-OH、i17 : 1ω9c、i17 : 0 3-OH以及总和特征3，包括i15 : 0 2-OH和/或16 : 1ω7c（总共占总量的76.2%）。MK-6是唯一的呼吸醌。不产生Flexirubin型色素。基于16S rRNA基因序列的系统发育分析表明，棘皮革兰氏菌KMM 6050T（该属唯一物种）是UST040801-001T的最密切亲缘关系菌，序列相似性为98.0%。UST040801-001T与棘皮革兰氏菌KMM 6050T之间的DNA-DNA相关性为13%。菌株UST040801-001T可通过11个表型特征与棘皮革兰氏菌区分开来。分子和表型分析结果表明，UST040801-001T代表革兰氏菌属的一个新物种。为此细菌提议命名为新种维多利亚港革兰氏菌（Gramella portivictoriae sp. nov.），以UST040801-001T（=NRRL B-41137T [校正后]=JCM 13192T）作为模式菌株。
