Li An-Zhang, Han Xi-Bin, Lin Long-Zhen, Zhang Ming-Xia, Zhu Hong-Hui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
Key Laboratory of Submarine Geosciences, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2018 Jan;68(1):358-363. doi: 10.1099/ijsem.0.002513. Epub 2017 Dec 5.
A Gram-stain-negative, aerobic, yellow-coloured, motile by gliding, rod-shaped bacterial strain, designated R17H11, was isolated from surface sediment collected from the Ross Sea, Antarctica. Growth optimally occurred at 25-30 °C, at pH 7.0-7.5 and in the presence of 3 % NaCl (w/v). Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R17H11 clustered together with Gramella flava JLT2011 and fell within the genus Gramella. Strain R17H11 shared the highest 16S rRNA gene similarities (96.1 and 96.0 %) with the type strains of Gramella forsetii and G. flava, and 92.6-95.5 % similarities with those of other known Gramella species. Strain R17H11 contained menaquinone-6 as the only isoprenoid quinone. The major fatty acids (>5 %) were summed feature 3 (17.5 %, comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 (14.0 %), summed feature 9 (11.8 %, comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c), iso-C17 : 0 3-OH (11.8 %), iso-C16 : 0 (7.4 %), C17 : 1ω6c (6.9 %) and anteiso-C15 : 0 (5.1 %). The major polar lipids were phosphatidylethanolamine, four unidentified lipids, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified glycolipid. The DNA G+C content of strain R17H11 was 38.6 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic characteristics, strain R17H11 represents a novel species in the genus Gramella, for which the name Gramellaantarctica sp. nov. is proposed. The type strain of the novel species is R17H11 (=GDMCC 1.1208=KCTC 52925).
从南极罗斯海采集的表层沉积物中分离出一株革兰氏染色阴性、需氧、黄色、能滑动、杆状的细菌菌株，命名为R17H11。该菌株在25 - 30 °C、pH 7.0 - 7.5以及3 % NaCl（w/v）条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株R17H11与黄褐海杆菌JLT2011聚在一起，属于海杆菌属。菌株R17H11与福氏海杆菌和黄褐海杆菌的模式菌株16S rRNA基因相似度最高（分别为96.1%和96.0 %），与其他已知海杆菌属物种的相似度为92.6 - 95.5 %。菌株R17H11仅含有甲基萘醌-6作为唯一的类异戊二烯醌。主要脂肪酸（>5 %）为总和特征3（17.5 %，包括C16 : 1ω7c和/或C16 : 1ω6c）、异C15 : 0（14.0 %）、总和特征9（11.8 %，包括10-甲基C16 : 0和/或异C17 : 1ω9c）、异C17 : 0 3-OH（11.8 %）、异C16 : 0（7.4 %）、C17 : 1ω6c（6.9 %）和anteiso-C15 : 0（5.1 %）。主要极性脂类为磷脂酰乙醇胺、四种未鉴定的脂类、一种未鉴定的氨基脂类、一种未鉴定的氨基磷脂类和一种未鉴定的糖脂类。菌株R17H11的DNA G+C含量为38.6 mol%。基于系统发育、生理和化学分类特征，菌株R17H11代表海杆菌属中的一个新物种，为此提出新名称南极海杆菌（Gramella antarctica sp. nov.）。该新物种的模式菌株为R17H11（=GDMCC 1.1208=KCTC 52925）。
