Feldstein M J, Golden J P, Rowe C A, Maccraith B D, Ligler F S
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375-5348, USA.
Biomed Microdevices. 1999;1(2):139-53. doi: 10.1023/A:1009900608757.
Optical and fluidics systems have been developed as central components for an automated array biosensor. Disposable planar waveguides are patterned with immobilized capture antibodies using a physically isolated patterning (PIP) method. The PIP method enables simultaneous deposition of several antibodies and completely circumvents cross-immobilization problems encountered with other array deposition processes. A multi-channel fluidics cell allows numerous assays to be performed on the patterned waveguide. The sensing arrays are optically interrogated using a diode laser with a tailored output to optimize coupling to and maximize excitation uniformity within the waveguide. A patterned cladding is employed to optically isolate the waveguide from perturbations induced by the permanently attached flow cells. Compact optics image the evanescently excited fluorescence onto a large area, cooled CCD array. The image data is processed and automated signal analysis corrects for local background and noise variations.
光学和流体系统已被开发为自动阵列生物传感器的核心组件。使用物理隔离图案化（PIP）方法，在一次性平面波导上固定捕获抗体进行图案化。PIP方法能够同时沉积多种抗体，并完全避免了其他阵列沉积过程中遇到的交叉固定问题。多通道流体池允许在图案化波导上进行大量检测。使用具有定制输出的二极管激光器对传感阵列进行光学询问，以优化与波导的耦合并使波导内的激发均匀性最大化。采用图案化包层将波导与永久连接的流通池引起的扰动进行光学隔离。紧凑的光学系统将倏逝激发荧光成像到大面积的冷却电荷耦合器件（CCD）阵列上。对图像数据进行处理，自动信号分析可校正局部背景和噪声变化。
