Taitt Chris Rowe, Shubin Yura S, Angel Roselina, Ligler Frances S
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C. 20375, USA.
Appl Environ Microbiol. 2004 Jan;70(1):152-8. doi: 10.1128/AEM.70.1.152-158.2004.
The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 x 10(4) CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross-reactivity tests were performed with Escherichia coli and Campylobacter jejuni. To determine whether the MAAB has potential as a screening tool for the diagnosis of asymptomatic Salmonella infection of poultry, chicken excretal samples from a private, noncommercial farm and from university poultry facilities were tested. While the private farm excreta gave rise to signals significantly above the buffer blanks, none of the university samples tested positive for S. enterica serovar Typhimurium without spiking; dose-response curves of spiked excretal samples from university-raised poultry gave limits of detection of 8 x 10(3) CFU/g.
多分析物阵列生物传感器（MAAB）是一种能够同时检测多种分析物的快速分析仪器。开发了用于检测肠炎沙门氏菌鼠伤寒血清型的快速（15分钟）单分析物夹心免疫测定法，检测限为8×10⁴CFU/ml；通过将测定方案延长至1小时，检测限提高了10倍。在以下加标食品中也检测到了肠炎沙门氏菌鼠伤寒血清型，样品制备最少：香肠、哈密瓜、全蛋液、苜蓿芽和鸡胴体冲洗液。对大肠杆菌和空肠弯曲菌进行了交叉反应试验。为了确定MAAB是否有潜力作为诊断家禽无症状沙门氏菌感染的筛查工具，对来自私人非商业农场和大学家禽设施的鸡排泄物样本进行了检测。虽然私人农场的排泄物产生的信号明显高于缓冲液空白，但未经加标的大学样本中没有一个检测出肠炎沙门氏菌鼠伤寒血清型呈阳性；来自大学饲养家禽的加标排泄物样本的剂量反应曲线给出的检测限为8×10³CFU/g。
