Wei Candong, Yang Jian, Zhu Junping, Zhang Xiaobing, Leng Wenchuan, Wang Jing, Xue Ying, Sun Lilian, Li Weijun, Wang Jin, Jin Qi
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, CAMS, Beijing 100730, Peoples Republic of China.
J Proteome Res. 2006 Aug;5(8):1860-5. doi: 10.1021/pr0601741.
Shigella flexneri is the causative agent of most shigellosis cases in developing countries. We used different proteolytic enzymes to selectively shave the protruding proteins on the surface of purified bacterial membrane sheets or vesicles, and recovered peptides were subsequently identified using 2-D LC-MS/MS. As a result, a total of 666 proteins were unambiguously assigned, including 159 integral membrane proteins, 35 outer membrane proteins and 114 proteins previously annotated as hypothetical. The former had an average grand average hydrophobicity score of 0.362 and were predicted to separate within a pH range of 4.1-10.6 with molecular mass 8-148 kDa, which represents the largest validated set of integral membrane proteins in this organism to date. A functional classification revealed that a large proportion of the identified proteins were involved in cell envelope biogenesis and energy production and conversion. For the first time, this work provides a global view of the S. flexneri 2a membrane subproteome.
福氏志贺菌是发展中国家大多数志贺氏菌病病例的病原体。我们使用不同的蛋白水解酶选择性地去除纯化的细菌膜片或囊泡表面突出的蛋白质，随后使用二维液相色谱-串联质谱法鉴定回收的肽段。结果，共明确鉴定出666种蛋白质，包括159种整合膜蛋白、35种外膜蛋白和114种先前注释为假设性的蛋白质。前者的平均总平均疏水性评分为0.362，预计在pH值4.1 - 10.6范围内分离，分子量为8 - 148 kDa，这是迄今为止该生物体中最大的一组经过验证的整合膜蛋白。功能分类显示，很大一部分鉴定出的蛋白质参与细胞包膜生物合成以及能量产生和转换。这项工作首次提供了福氏志贺菌2a膜亚蛋白质组的全局视图。
