Naziroğlu Mustafa
Department of Physiology, Veterinary Faculty, Firat University, TR-23119 Elaziğ, Turkey.
Biol Trace Elem Res. 2003 Jul;94(1):61-72. doi: 10.1385/BTER:94:1:61.
Diabetes mellitus is associated with diabetic impairment of testicular function, ultimately leading to reduced fertility. Its etiology may involve oxidative damage by reactive oxygen substances, and protection against this damage can be offered by antioxidant supplementation. The aim of this study was to investigate the effects of intraperitoneal administration of vitamin C and E, selenium (Se), and vitamin E plus Se (COM) on concentrations of lipid peroxide (as malondialdehyde; MDA), reduced glutathione (GSH), and vitamin E concentrations and glutathione peroxidase (GSH-Px) activity in the testes of rats with diabetes induced by streptozotocin (STZ). Sixty groups were used (10 animals each) and these animals were initially allocated to two groups: control group and diabetic group. The diabetic group was subdivided into five groups as follows: diabetic control (DC), vitamin E, Se, COM, and vitamin C. Animals in the DC group and vitamin C, vitamin E, Se, and COM groups were made diabetic by the injection of STZ on 4 d after an injection of vitamins C and E, Se, and COM. Those vitamins and Se were also administered for 21 consecutive days. The MDA, vitamin E, GSH levels, and GSH-Px activities in testes were determined. Although the vitamin E concentration was higher in the control than in the DC group, the MDA levels were found to be lower in the control than in the DC group. The MDA levels in the testes samples of vitamin C, vitamin E, Se, and COM groups were lower than the DC group. However, GSH-Px activity and GSH levels in the testes were not significantly different between the control and DC groups. Vitamin E concentrations in the vitamin C, vitamin E, Se, and COM groups and GSH levels and GSH-Px activities in the Se, COM, and vitamin C groups were higher than either the control or DC group. The results indicate that reactive oxygen substances may be involved in possible testicular complications in diabetes of rats. Administration of vitamins C and E and Se reduced the testicular lipid peroxidation; these vitamins and Se had significant protective effects on testes of rats against oxidative damage in diabetes.
糖尿病与睾丸功能的糖尿病性损害相关，最终导致生育能力下降。其病因可能涉及活性氧物质的氧化损伤，而补充抗氧化剂可提供针对这种损伤的保护。本研究的目的是探讨腹腔注射维生素C、维生素E、硒（Se）以及维生素E加硒（COM）对链脲佐菌素（STZ）诱导的糖尿病大鼠睾丸中脂质过氧化物（以丙二醛；MDA表示）浓度、还原型谷胱甘肽（GSH）浓度、维生素E浓度以及谷胱甘肽过氧化物酶（GSH-Px）活性的影响。使用了60只大鼠（每组10只），这些动物最初被分为两组：对照组和糖尿病组。糖尿病组又细分为五组，如下：糖尿病对照组（DC）、维生素E组、硒组、COM组和维生素C组。DC组以及维生素C、维生素E、硒和COM组的动物在注射维生素C、维生素E、硒和COM 4天后通过注射STZ诱导糖尿病。这些维生素和硒也连续给药21天。测定睾丸中的MDA、维生素E、GSH水平以及GSH-Px活性。虽然对照组的维生素E浓度高于DC组，但发现对照组的MDA水平低于DC组。维生素C、维生素E、硒和COM组睾丸样本中的MDA水平低于DC组。然而，对照组和DC组睾丸中的GSH-Px活性和GSH水平没有显著差异。维生素C、维生素E、硒和COM组的维生素E浓度以及硒、COM和维生素C组的GSH水平和GSH-Px活性高于对照组或DC组。结果表明，活性氧物质可能参与大鼠糖尿病中可能出现的睾丸并发症。维生素C、维生素E和硒的给药减少了睾丸脂质过氧化；这些维生素和硒对糖尿病大鼠的睾丸免受氧化损伤具有显著的保护作用。
