Zadvorny Oleg A, Zorin Nikolay A, Gogotov Ivan N
Institute of Basic Biological Problems of RAS, 142290 Pushchino, Russia.
Arch Microbiol. 2006 Jan;184(5):279-85. doi: 10.1007/s00203-005-0040-1. Epub 2005 Nov 11.
The ability of hydrogenases isolated from Thiocapsa roseopersicina and Lamprobacter modestohalophilus to reduce metal ions and oxidize metals has been studied. Hydrogenases from both phototrophic bacteria oxidized metallic Fe, Cd, Zn and Ni into their ionic forms with simultaneous evolution of molecular hydrogen. The metal oxidation rate decreased in the series Zn > Fe > Cd > Ni and depended on the pH. The presence of methyl viologen in the reaction system accelerated this process. T. roseopersicina and L. modestohalophilus cells and their hydrogenases reduced Ni(II), Pt(IV), Pd(II) or Ru(III) to their metallic forms under H2 atmosphere. These results suggest that metals or metal ions can serve as electron donors or acceptors for hydrogenases from phototrophic bacteria.
对从玫瑰色硫囊菌和嗜盐温和发光杆菌中分离出的氢化酶还原金属离子和氧化金属的能力进行了研究。来自这两种光合细菌的氢化酶将金属铁、镉、锌和镍氧化成离子形式，同时释放出分子氢。金属氧化速率在锌>铁>镉>镍的序列中降低，并且取决于pH值。反应体系中甲基紫精的存在加速了这一过程。在氢气氛围下，玫瑰色硫囊菌和嗜盐温和发光杆菌细胞及其氢化酶将镍（II）、铂（IV）、钯（II）或钌（III）还原为金属形式。这些结果表明，金属或金属离子可以作为光合细菌氢化酶的电子供体或受体。
