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莱氏野村菌对家蚕侵袭致绿僵病的扫描电镜研究

Sem study on the invasion of Nomuraea rileyi (Farlow) on silkworm, Bombyx mori Linn. causing green muscardine.

作者信息

Kumar V, Singh G P, Kumar V, Babu A M, Datta R K

机构信息

Electron Microscopy Unit, Central Sericultural Research and Training Institute, Srirampura, Mysore-570 008, India.

出版信息

Mycopathologia. 1997;139(3):141-4. doi: 10.1023/A:1006800231647.

DOI:10.1023/A:1006800231647
PMID:16283452
Abstract

The mature conidia of Nomuraea rileyi (Farlow) germinate on the larval integument of Bombyx mori within 24 h and penetrate the cuticle within 36 h after inoculation at 24.0 +/- 1.0 degrees C temperature and 80.0 +/- 5.0% relative humidity. The penetrating hyphae multiply by budding and septa formation in the hemocoel, and the larva succumbs to the infection 6-7 days post-treatment. The hyphal bodies elongate and become interwoven with other hyphae forming a mycelial complex across different tissues. The ramification of hyphae along the epidermal tissue results in larval mummification in 7-8 days. Numerous conidiophores emerge, producing a confluent white fungal mat over the entire surface of the host larva by 9-10 days. Pale green conidia develop, making the larval body green. Life cycle of the fungus on B. mori is completed in 10-11 days.

摘要

在温度24.0±1.0摄氏度、相对湿度80.0±5.0%的条件下,莱氏野村菌(Farlow)的成熟分生孢子在24小时内在家蚕幼虫体表萌发,并在接种后36小时内穿透表皮。穿透的菌丝在血腔中通过出芽和隔膜形成进行增殖,幼虫在处理后6 - 7天死于感染。菌丝体伸长并与其他菌丝交织,在不同组织中形成菌丝复合体。沿着表皮组织的菌丝分支导致幼虫在7 - 8天内被木乃伊化。到9 - 10天时,大量分生孢子梗出现,在宿主幼虫的整个表面形成一层汇合的白色真菌层。淡绿色分生孢子发育形成,使幼虫体呈绿色。该真菌在家蚕上的生命周期在10 - 11天内完成。

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引用本文的文献

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World J Microbiol Biotechnol. 2014 Jul;30(7):1927-35. doi: 10.1007/s11274-014-1610-7. Epub 2014 Feb 5.
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Phospholipase A₂ inhibitors in bacterial culture broth enhance pathogenicity of a fungus Nomuraea rileyi.
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J Microbiol. 2012 Aug;50(4):644-51. doi: 10.1007/s12275-012-2108-3. Epub 2012 Jul 21.
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