Noda Takahiro, Ono Masateru, Iimure Kazuhiko, Araki Tomohiro
Kumamoto Prefectural Agriculture Research Center, Koshi, Kumamoto, Japan.
Biosci Biotechnol Biochem. 2010;74(3):563-8. doi: 10.1271/bbb.90757. Epub 2010 Mar 7.
The conidium of the entomopathogenic fungus Nomuraea rileyi has been found to germinate rapidly in the presence of host insect-derived extracts. Thus the extract appears to contain an important factor involved in host recognition by N. rileyi. However, the substance responsible for such unique germination behavior has yet to be identified. Hence we attempted to purify this substance. One thousand g of dried silkworm pupae was subjected to methanol extraction, followed by methanolysis, two different solvent partitions, and three different column chromatographies. A total of 12.4 mg of substance was obtained in the active fraction. The substance obtained exhibited an activity more than 46,000 times higher than that of the methanol extract. The substance was detected as a single peak on Sephadex LH20 column chromatography and as a single band on high-performance thin-layer chromatography. These data indicate that the concentrated fraction contained a high-purity substance.
已发现昆虫病原真菌莱氏野村菌的分生孢子在存在宿主昆虫来源的提取物时会迅速萌发。因此，该提取物似乎含有莱氏野村菌识别宿主所涉及的重要因子。然而，导致这种独特萌发行为的物质尚未被鉴定出来。因此，我们试图纯化这种物质。将1000克干蚕蛹进行甲醇提取，随后进行甲醇解、两种不同的溶剂分配和三种不同的柱色谱法。在活性级分中总共获得了12.4毫克物质。所获得的物质表现出比甲醇提取物高46000倍以上的活性。该物质在葡聚糖LH20柱色谱上检测为单一峰，在高效薄层色谱上检测为单一带。这些数据表明浓缩级分含有高纯度物质。
