Mo Zhihong, Wang Hongli, Liang Yulong, Liu Fengwei, Xue Yongxin
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, People's Republic of China.
Analyst. 2005 Dec;130(12):1589-94. doi: 10.1039/b500949a. Epub 2005 Oct 3.
A nanoparticle-bioconjugate was formed by homogeneous hybridization of one polynucleotide target with two oligonucleotide probes labelled by thiol and a nanoparticle, respectively. Deposition of the nanoparticle-bioconjugate on a gold surface by thiol-gold reaction was monitored in situ by quartz crystal microbalance (QCM) and applied for flow analysis of zeptomole amounts of polynucleotide. The formation in solution and adsorption of thiolated conjugates on gold could be fast, uniform and effective, and has been successfully exploited to construct a highly reproducible and sensitive platform for detection of target sequences. Being more rapid, reproducible, sensitive and amenable to automation than previously reported microgravimetric hybridization assays, this technology has great promise for practical applications in molecular diagnostics.
通过一种多核苷酸靶标与分别用硫醇和纳米颗粒标记的两种寡核苷酸探针进行均相杂交，形成了纳米颗粒生物共轭物。利用石英晶体微天平（QCM）对纳米颗粒生物共轭物通过硫醇-金反应沉积在金表面的过程进行原位监测，并将其应用于zeptomole量多核苷酸的流动分析。硫醇化共轭物在溶液中的形成及其在金上的吸附可以快速、均匀且有效地进行，并且已成功用于构建用于检测靶序列的高度可重复且灵敏的平台。与先前报道的微重力杂交分析相比，该技术更快、更可重复、更灵敏且易于自动化，在分子诊断的实际应用中具有很大的前景。
