Martiniaková M, Omelka R, Chrenek P, Ryban L, Parkányi V, Grosskopf B, Vondráková M, Bauerová M
Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia.
Folia Biol (Praha). 2005;51(5):140-4.
Bone tissue microstructure of femur was investigated in transgenic New Zealand White rabbits with human factor VIII gene. Altogether 42 bones (24 from transgenic rabbits and 18 from non-transgenic ones) were analysed. Specimens were prepared using standard histological equipment, producing thin sections of approximately 80-100 microm. For histomorphometrical analysis areas, perimeters, minimum and maximum diameters of osteons' vascular canals and of osteons were measured. We found out that the basic structural pattern of femoral bone tissue was primary vascular longitudinal in both groups of rabbits. However, a new type of the bone tissue--fibrolamellar--was identified only in the transgenic rabbits. The measured variables of the osteons' vascular canals were higher in transgenic individuals in comparison with the nontransgenic ones (except for maximal diameter) and the differences were statistically significant (P < 0.05; P < 0.01). We suppose that the observed differences could be associated with transgenesis. In an effort to explain these differences we compared the cytogenetic profile of bone marrow cells between transgenic and non-transgenic rabbits. A significantly higher rate of aneuploidy was observed in c-metaphase spreads of transgenic individuals as compared to non-transgenic ones (P < 0.001). Despite the fact that no hFVIII mRNA expression was found in the femur of transgenic rabbits, we discussed an association of transgene integration into the genome and microstructural changes in the bone. In any case, the results indicate that transgenesis can also produce changes in other tissues than in the target ones.
在携带人因子VIII基因的转基因新西兰白兔中，对股骨的骨组织微观结构进行了研究。总共分析了42块骨头（24块来自转基因兔，18块来自非转基因兔）。使用标准组织学设备制备标本，制成约80 - 100微米的薄片。对于组织形态计量学分析，测量了骨单位血管腔和骨单位的面积、周长、最小和最大直径。我们发现，两组兔子股骨骨组织的基本结构模式均为初级血管纵向模式。然而，仅在转基因兔中发现了一种新型的骨组织——纤维板层骨。与非转基因个体相比，转基因个体的骨单位血管腔测量变量更高（最大直径除外），差异具有统计学意义（P < 0.05；P < 0.01）。我们推测观察到的差异可能与转基因有关。为了解释这些差异，我们比较了转基因兔和非转基因兔骨髓细胞的细胞遗传学图谱。与非转基因个体相比，转基因个体的c中期涂片观察到明显更高的非整倍体率（P < 0.001）。尽管在转基因兔的股骨中未发现hFVIII mRNA表达，但我们讨论了转基因整合到基因组与骨微观结构变化之间的关联。无论如何，结果表明转基因也可在目标组织以外的其他组织中产生变化。
