Curlej Jozef, Parkanyi Vladimir, Bulla Jozef, Jurcik Rastislav, Chrenek Peter
Slovak Agricultural Research Centre, Hlohovska 2, 949 92 Nitra, Slovak Republic.
Folia Biol (Krakow). 2007;55(3-4):161-4. doi: 10.3409/173491607781492614.
The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no. 1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 microg/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.
本研究的目的是比较F4代转基因兔和非转基因兔之间的染色体非整倍体率。对具有不同遗传背景的新西兰白兔转基因（携带人凝血因子VIII基因）和非转基因兔（F4代）的骨髓样本进行了染色体分析（雌性1-3-5系I和雌性1-9-7系II）。通过添加0.25μg/ml秋水仙酰胺使骨髓淋巴细胞同步化，获得C中期板。在I系转基因兔（61%）和非转基因兔（51.27%）之间未观察到染色体非整倍体的显著差异。另一方面，在II系转基因兔和非转基因兔之间发现非整倍体率较高但不显著，而在二倍体率方面观察到显著差异（P<0.05）。总之，本实验中的染色体非整倍体率高于先前其他报告中公布的结果。
