Kajiwara Hiroshi, Yasuda Masanori, Kumaki Nobue, Shibayama Taro, Osamura Yoshiyuki
Department of Pathology, Tokai University School of Medicine, Bouseidai, Isehara, Kanagawa 259-1193, Japan.
Tokai J Exp Clin Med. 2005 Sep;30(3):177-82.
Carbohydrate antigens and E-selectin play important roles in the invasion and metastasis of cancers. We examined the expression of these antigens and their ligand protein, E-selectin, in urothelial carcinomas to evaluate whether their staining is correlated with the grade and stage of cancer. We studied the expression of carbohydrate antigens (type 1 and type 2 blood-group antigens) and E-selectin in urothelial carcinomas of the renal pelvis, ureter, and urinary bladder in 52 patients by staining SSEA-1 (LeX), sialyl LeX (sLeX), DU-PAN-2, CA19-9, and E-selectin with 5 different monoclonal antibodies (MAbs) to evaluate whether their staining correlated with cancer grade and stage. The differences between organs with regard to the degree of expression of these antigens were not evident. Type 2 antigens (SSEA-1 and sialyl LeX) are frequently expressed in the tumor cells regardless of atypical grade. The expression level of type 1 antigens (DU-PAN-2 and CA19-9) is lower than that of type 2 antigens. However, the presence of DU-PAN-2 tends to correlate with the grade of atypia; however, that of CA19-9 is inversely proportional to the grade of atypia. The lack of CA19-9 and appearance of DU-PAN-2 in urothelial carcinoma implies a high malignant potential. The expression of E-selectin can be correlated with stage and grade of tumor atypia. Type 2 antigen and E-selectin may be involved in tumor invasion and metastasis.
碳水化合物抗原和E选择素在癌症的侵袭和转移中发挥着重要作用。我们检测了这些抗原及其配体蛋白E选择素在尿路上皮癌中的表达，以评估它们的染色是否与癌症的分级和分期相关。我们通过用5种不同的单克隆抗体（MAb）对SSEA-1（LeX）、唾液酸化LeX（sLeX）、DU-PAN-2、CA19-9和E选择素进行染色，研究了52例肾盂、输尿管和膀胱尿路上皮癌中碳水化合物抗原（1型和2型血型抗原）和E选择素的表达，以评估它们的染色是否与癌症分级和分期相关。这些抗原表达程度在不同器官之间的差异并不明显。2型抗原（SSEA-1和唾液酸化LeX）在肿瘤细胞中频繁表达，与非典型分级无关。1型抗原（DU-PAN-2和CA19-9）的表达水平低于2型抗原。然而，DU-PAN-2的存在往往与非典型分级相关；然而，CA19-9的存在与非典型分级呈负相关。尿路上皮癌中CA19-9的缺失和DU-PAN-2的出现意味着高恶性潜能。E选择素的表达可与肿瘤非典型性的分期和分级相关。2型抗原和E选择素可能参与肿瘤的侵袭和转移。
