Agudo David, Gómez-Esquer Francisco, Díaz-Gil Gema, Martínez-Arribas Fernando, Delcán José, Schneider José, Palomar María Angustias, Linares Rafael
Departamento de Ciencias de la Salud III, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Avenida de Atenas, S/N E28922 Alcorcón, Madrid, Spain.
Proteomics. 2005 Dec;5(18):4946-57. doi: 10.1002/pmic.200402056.
The chicken (Gallus gallus) is one of the primary models for embryological and developmental studies. In order to begin to understand the molecular mechanisms underlying the normal and abnormal development of the chicken, we used 2-DE to construct a whole-embryo proteome map. Proteins were separated by IEF on IPG strips, and by 11% SDS-PAGE) gels. Protein identification was performed by means of PMF with MALDI-TOF-MS. In all, 105 protein spots were identified, 35 of them implicated in embryo development, 10 related with some diseases, and 16, finally, being proteins that have never been identified, purified or characterized in the chicken before. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level under different physiological conditions.
鸡（原鸡）是胚胎学和发育研究的主要模型之一。为了开始理解鸡正常和异常发育背后的分子机制，我们使用双向电泳构建了全胚胎蛋白质组图谱。蛋白质在IPG胶条上通过等电聚焦分离，并在11%十二烷基硫酸钠聚丙烯酰胺凝胶（SDS-PAGE）上进一步分离。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）的肽质量指纹图谱（PMF）进行蛋白质鉴定。总共鉴定出105个蛋白质斑点，其中35个与胚胎发育有关，10个与某些疾病相关，最后16个是此前从未在鸡中鉴定、纯化或表征过的蛋白质。该图谱将不断更新，并将作为研究人员的参考数据库，用于研究不同生理条件下蛋白质水平的变化。
