UCD Conway Institute of Biomolecular and Biomedical Research, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
Proteomics. 2011 Sep;11(17):3582-6. doi: 10.1002/pmic.201000182. Epub 2011 Aug 4.
We have undertaken the identification of basic proteins (pH 6-11) of the human heart using 2-DE. Tissue from the left ventricle of human heart was lysed and proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading followed by separation on 12% SDS polyacrylamide gels in the second dimension. Proteins were then identified by mass spectrometry and analysed using Proline, a proteomic data analysis platform that was developed in-house. The proteome map contains 176 identified spots with 151 unique proteins and has been made available as part of the UCD-2DPAGE database at http://proteomics-portal.ucd.ie:8082. The associated mass spectrometry data have been submitted to PRIDE (Accession number ♯10098). This reference map, and the other heart reference maps available through the UCD-2DPAGE database, will aid further proteomic studies of heart diseases such as dilated cardiomyopathy and ischaemic heart disease.
我们使用 2-DE 技术对人心肌中的基本蛋白（pH6-11）进行了鉴定。用人左心室组织进行裂解，在第一维通过纸桥加载在 pH6-11 的 IPG 条上进行分离，然后在第二维上通过 12% SDS 聚丙烯酰胺凝胶进行分离。通过质谱对蛋白质进行鉴定，并使用 Proline（一个内部开发的蛋白质组数据分析平台）进行分析。蛋白质组图谱包含 176 个鉴定斑点，其中 151 个为独特蛋白质，并且已作为 UCD-2DPAGE 数据库的一部分在 http://proteomics-portal.ucd.ie:8082 上提供。相关的质谱数据已提交给 PRIDE（注册号♯10098）。该参考图谱以及 UCD-2DPAGE 数据库中提供的其他心脏参考图谱，将有助于进一步研究扩张型心肌病和缺血性心脏病等心脏疾病的蛋白质组学。
