Ding Ren, McGuinness Cathy L, Burnand Kevin G, Sullivan Elizabeth, Smith Alberto
Academic Department of Surgery, King's College, St Thomas' Campus, London, UK.
Vascular. 2005 Sep-Oct;13(5):290-7. doi: 10.1258/rsmvasc.13.5.290.
The purpose of this study was to determine the effect of low-dose doxycycline on matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP)-1 expression in the wall of abdominal aortic aneurysms. A double-blind, randomized study was conducted of patients treated with doxycycline (100 mg/d orally) or placebo for 1 month prior to surgery. MMP-2, -3, and -9 (zymogen and activity); MMP-1, -2, -3, -7, -9, -11, -12, and -14; and TIMP-1 (messenger ribonucleic acid [mRNA]) were measured in the aneurysm wall. No differences were found between the treatment and placebo groups in zymogen levels of MMP-2, -3, or -9 or in the free or total activities of MMP-2 and -9. Treatment with doxycycline also had no effect on the concentration of any mRNA measured. No relationship was found between the number of tablets taken and MMP or TIMP protein, mRNA, or activity levels in the aneurysm wall. Low-dose doxycycline treatment does not alter the expression or activity of metalloproteinases or their inhibitor, TIMP-1, in the aneurysm wall.
本研究的目的是确定低剂量强力霉素对腹主动脉瘤壁中基质金属蛋白酶（MMP）和金属蛋白酶组织抑制剂（TIMP）-1表达的影响。在手术前1个月，对接受强力霉素（100mg/d口服）或安慰剂治疗的患者进行了一项双盲随机研究。测量了动脉瘤壁中的MMP-2、-3和-9（酶原和活性）；MMP-1、-2、-3、-7、-9、-11、-12和-14；以及TIMP-1（信使核糖核酸[mRNA]）。在MMP-2、-3或-9的酶原水平以及MMP-2和-9的游离或总活性方面，治疗组和安慰剂组之间未发现差异。强力霉素治疗对所测量的任何mRNA浓度也没有影响。在所服用片剂数量与动脉瘤壁中MMP或TIMP蛋白、mRNA或活性水平之间未发现相关性。低剂量强力霉素治疗不会改变动脉瘤壁中金属蛋白酶或其抑制剂TIMP-1的表达或活性。
