Borges Erika C, Machado Evandro M M, Garcia Eloi S, Azambuja Patrícia
Laboratório de Bioquímica e Fisiologia de Insetos, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil 4365, CEP 21045-900, Rio de Janeiro, RJ, Brazil.
Exp Parasitol. 2006 Feb;112(2):130-3. doi: 10.1016/j.exppara.2005.09.008. Epub 2005 Nov 8.
Cathepsin D activity was estimated in midgut homogenates from Rhodnius prolixus, uninfected and experimentally infected with Trypanosoma cruzi, at different times after blood ingestion. No enzyme activity was found in the anterior midgut and rectum. In the posterior midgut, enzyme activity was found both in lumen and wall. In starved uninfected insects, in lumen and wall, cathepsin D activity was high, decreasing to a constant rate at 1-15 days after feeding. In insects infected with T. cruzi cathepsin D activity increased 1 and 3 days after blood meal. We suggest that these changes in cathepsin D activity in R. prolixus posterior midgut are due to the establishment of T. cruzi infection.
在不同的取食后时间，对未感染和经克氏锥虫实验感染的长红猎蝽中肠匀浆中的组织蛋白酶D活性进行了评估。在前肠和直肠中未发现酶活性。在后肠中，酶活性在肠腔和肠壁中均有发现。在饥饿的未感染昆虫中，肠腔和肠壁中的组织蛋白酶D活性较高，在取食后1 - 15天以恒定速率下降。在感染克氏锥虫的昆虫中，取食后1天和3天组织蛋白酶D活性增加。我们认为，长红猎蝽后肠中组织蛋白酶D活性的这些变化是由于克氏锥虫感染的建立。
