Mortensen M W, Sørensen P G, Björkdahl O, Jensen M R, Gundersen H J G, Bjørnholm T
Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
Appl Radiat Isot. 2006 Mar;64(3):315-24. doi: 10.1016/j.apradiso.2005.08.003. Epub 2005 Nov 15.
Boron carbide nanoparticles are proposed as a system for T cell-guided boron neutron capture therapy. Nanoparticles were produced by ball milling in various atmospheres of commercially available boron carbide. The physical and chemical properties of the particles were investigated using transmission electron microscopy, photon correlation spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction, vibrational spectroscopy, gel electrophoresis and chemical assays and reveal profound changes in surface chemistry and structural characteristics. In vitro thermal neutron irradiation of B16 melanoma cells incubated with sub-100 nm nanoparticles (381.5 microg/g (10)B) induces complete cell death. The nanoparticles alone induce no toxicity.
碳化硼纳米颗粒被提议作为一种用于T细胞引导的硼中子俘获疗法的系统。通过在各种气氛中对市售碳化硼进行球磨来制备纳米颗粒。使用透射电子显微镜、光子相关光谱、X射线光电子能谱、X射线衍射、振动光谱、凝胶电泳和化学分析对颗粒的物理和化学性质进行了研究，结果显示其表面化学和结构特征发生了深刻变化。用亚100纳米纳米颗粒（381.5微克/克（10）B）孵育的B16黑色素瘤细胞进行体外热中子照射可导致细胞完全死亡。单独的纳米颗粒不会诱导毒性。
