Total anterior corneal surface and epithelial stem cell harvesting: current microkeratomes and beyond.

Currently, most corneal lamellar allografts are performed by manually dissecting donor corneoscleral material for transplantation. Manual dissection is technically challenging and time intensive, and excessive handling of tissue may decrease the likelihood of obtaining viable grafts. These mechanical factors, along with problems controlling immune rejection, have prevented limbal stem cell lamellar allografts in particular from becoming more widely used. Anterior lamellar keratoplasty is gaining in clinical importance and usage but is still only practiced by a limited number of surgeons. Recently, new mechanical and femtosecond laser microkeratomes have been adapted to harvest the anterior corneal surface, including the limbal stem cell region. Preliminary results have been encouraging and give hope that this technique may prove to be of benefit to patients in the future.