Mózsik G, Sütö G, Vincze A
First Department of Medicine, Medical University of Pécs, Hungary.
J Clin Gastroenterol. 1992;14 Suppl 1:S135-9. doi: 10.1097/00004836-199206001-00024.
The aims of the present investigation were to compare the acute chemical (atropine given in a dose of 1.0 mg/kg s.c.) and bilateral surgical vagotomy-induced biochemical changes in the rat gastric mucosa. The biochemical measurements were carried out at 1.5 h after administration of atropine and surgical vagotomy. The adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and lactate were measured enzymatically, and cyclic adenosine monophosphate (cAMP) was determined by RIA, from the total homogenate of gastric mucosa. The ratio of ATP/ADP, values of the adenylate pool (ATP + ADP + AMP), and energy charge [(ATP + 0.5 ADP)/(ATP + ADP + AMP)] were calculated. It was found that (a) no significant changes were obtained in the gastric mucosal levels of ATP, ADP, AMP, adenylate pool, cAMP, lactate, and ratio of ATP/ADP, but were with energy charge, which decreased in rats with acute surgical vagotomy; (b) the tissue levels of ATP, ADP, ratio of ATP/ADP, AMP, and adenylate pool were decreased, while the energy charge and cAMP increased in the atropine-treated group; and (c) significant differences were obtained between the chemical and surgical vagotomy-induced changes in the tissue levels of ATP, ADP, ratio of ATP/ADP, AMP, adenylate pool, energy charge, and cAMP. It is concluded that the chemical and surgical vagotomy-induced biochemical changes in the gastric mucosa differed significantly at 1.5 h after administration of atropine or surgical vagotomy, but no elevation was obtained in the gastric mucosal level of lactate; and chemical vagotomy regulates the membrane-bound ATP-dependent energy systems, but this regulation does not occur after surgical vagotomy.
本研究的目的是比较急性化学性（皮下注射剂量为1.0mg/kg的阿托品）和双侧手术切断迷走神经引起的大鼠胃黏膜生化变化。在给予阿托品和进行手术切断迷走神经后1.5小时进行生化测量。用酶法测定胃黏膜总匀浆中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）和乳酸，用放射免疫分析法测定环磷酸腺苷（cAMP）。计算ATP/ADP比值、腺苷酸库（ATP+ADP+AMP）值和能荷[（ATP+0.5ADP）/（ATP+ADP+AMP）]。结果发现：（a）胃黏膜中ATP、ADP、AMP、腺苷酸库、cAMP、乳酸水平及ATP/ADP比值无显著变化，但能荷有变化，急性手术切断迷走神经的大鼠能荷降低；（b）阿托品处理组的ATP、ADP、ATP/ADP比值、AMP和腺苷酸库组织水平降低，而能荷和cAMP升高；（c）化学性和手术切断迷走神经引起的ATP、ADP、ATP/ADP比值、AMP、腺苷酸库、能荷和cAMP组织水平变化存在显著差异。结论是，在给予阿托品或进行手术切断迷走神经后1.5小时，化学性和手术切断迷走神经引起的胃黏膜生化变化有显著差异，但胃黏膜乳酸水平未升高；化学性切断迷走神经可调节膜结合的ATP依赖性能量系统，但手术切断迷走神经后则无此调节作用。
