Lippi U, Cagnin V
Department of Pathology, P. Cosma Hospital, Camposampiero, Italy.
Biochem Exp Biol. 1977;13(4):425-9.
An automated procedure for the determination of serum gamma-glutamyltranspeptidase using Technicon SMAC alkaline phosphatase flow channel has been developed. The flow variants are insignificant. We use L-gamma-glutamyl-3-carboxy-4-nitroanilide as a very soluble substrate. With the dyalisis procedure, hemoglobin and bilirubin do not interfere. By employing dialysis, the need of blank corrections is therefore eliminated. The procedure can been run at 130 determinations per hour with insignificant carryover and satisfactory precision.
已开发出一种利用Technicon SMAC碱性磷酸酶流动通道测定血清γ-谷氨酰转肽酶的自动化程序。流动变量微不足道。我们使用L-γ-谷氨酰-3-羧基-4-硝基苯胺作为极易溶解的底物。通过透析程序，血红蛋白和胆红素不会产生干扰。因此，采用透析消除了空白校正的需求。该程序每小时可进行130次测定，残留量微不足道，精密度令人满意。
