Pinheiro Sérgio Luiz, Simionato Maria Regina Lorenzetti, Imparato José Carlos Pettorossi, Oda Margareth
Dental School, University of São Paulo, Brazil.
Am J Dent. 2005 Aug;18(4):261-6.
To assess the total viable bacteria in infected dentin after sealing with glass-ionomer cement containing 1% metronidazole, 1% ciprofloxacin and 1% cefaclor.
40 children were selected, with ages ranging from 4-10 years, with primary teeth with active carious lesions without any pulpal involvement. The patients were divided randomly into two groups in accordance to the glass-ionomer cement used to seal the carious lesions: Group 1 (control, n=20), sealing with conventional glass-ionomer cement; and Group 2 (test, n=20) sealing with glassionomer cement including antibiotics. The infected dentin was collected both before and 24 hours after the sealing of the cavities and, after inoculation in blood agar, all the plates were incubated in containers kept at 37 degrees C for 5 days in an atmosphere of 85% N2, 10% CO2 and 5% H2, for subsequent counting of total viable bacteria.
The results were analyzed using the Wilcoxon and Mann-Whitney tests. The glass-ionomer cement with 1% of metronidazole, 1% of ciprofloxacin and 1% of cefaclor showed a significantly greater reduction in microbiota in the infected dentin in comparison to the reduction with the conventional ionomer cement (P< 0.01), with a mean reduction of 98.65% of all viable bacteria. The infected dentin after sealing with glass-ionomer cement with antibiotics showed, using scanning electron microscopy, the presence of bacterial aggregates, intertubular dentin with exposure of collagen fibers, and dentin tubules.
评估用含1%甲硝唑、1%环丙沙星和1%头孢克洛的玻璃离子水门汀封闭后感染牙本质中的总活菌数。
选取40名年龄在4至10岁之间、患有原发性牙齿活动性龋损且无任何牙髓受累的儿童。根据用于封闭龋损的玻璃离子水门汀将患者随机分为两组：第1组（对照组，n = 20），用传统玻璃离子水门汀封闭；第2组（试验组，n = 20），用含抗生素的玻璃离子水门汀封闭。在窝洞封闭前和封闭后24小时收集感染牙本质，接种于血琼脂后，将所有平板置于37℃的容器中，在85% N2、10% CO2和5% H2的气氛中培养5天，随后对总活菌数进行计数。
采用Wilcoxon和Mann-Whitney检验分析结果。含1%甲硝唑、1%环丙沙星和1%头孢克洛的玻璃离子水门汀与传统离子水门汀相比，感染牙本质中的微生物群减少更为显著（P < 0.01），所有活菌的平均减少率为98.65%。用含抗生素的玻璃离子水门汀封闭后的感染牙本质，通过扫描电子显微镜观察，可见细菌聚集体、胶原纤维暴露的管间牙本质和牙本质小管。
