Kim Jungdae, Choi Chunho, Lim Jaekwan, You Hyeran, Sim Sung-Bo, Yom Yoon-Ki, Kim Eui-Han, Soh Kwang-Sup
Biomedical Physics Laboratory for Korean Medicine, School of Physics, Seoul National University, Seoul, Republic of Korea.
J Altern Complement Med. 2005 Oct;11(5):879-84. doi: 10.1089/acm.2005.11.879.
The aim of this study was to measure spontaneous photon emission (SPE) and delayed luminescence (DL) from various human cancer tissues.
A photomultiplier tube attached to a dark chamber was used for the detection of ultraweak photon emission from cancer tissues in the chamber. The samples were illuminated with a 150 W metal halide lamp for the measurement of delayed luminescence. Frozen tissues were provided by the hospitals and preserved in saline solution in a CO2 incubator for 1 hour before starting the measurement of spontaneous photon emission. We successively measured the afterglows from the samples after 30-second irradiation of the lamp. The samples were divided into two groups: tumor tissues and normal tissues around tumor tissues. We presented experimental data and interpreted their characteristic patterns of spontaneous photon emission and delayed luminescence.
Mean values of spontaneous photon emissions from the normal tissues and the tumor tissues were measured with the standard errors of the mean as 625 +/- 419 counts/minute/cm2 (n = 6) and 982 +/- 513 counts/minute/cm2 (n = 14), respectively. Peak values of the intensity of delayed luminescence from normal and cancerous tissues were 63 +/- 20 counts/ms (n = 6) and 48 +/- 12 counts/ms (n = 14).
The intensity of spontaneous photon emissions from cancer tissues were mostly discriminated from those of normal tissues, and their delayed luminescent properties were investigated.
本研究旨在测量各种人类癌症组织的自发光子发射（SPE）和延迟发光（DL）。
将一个连接到暗箱的光电倍增管用于检测箱内癌症组织的超微弱光子发射。用一盏150瓦的金属卤化物灯照射样本以测量延迟发光。样本由医院提供，为冷冻组织，在开始测量自发光子发射前，将其保存在生理盐水中并置于二氧化碳培养箱中1小时。在灯照射30秒后，我们连续测量样本的余辉。样本分为两组：肿瘤组织和肿瘤周围的正常组织。我们展示了实验数据，并解释了它们自发光子发射和延迟发光的特征模式。
正常组织和肿瘤组织自发光子发射的平均值分别测量为625±419计数/分钟/平方厘米（n = 6）和982±513计数/分钟/平方厘米（n = 14），平均值的标准误差分别为上述值。正常组织和癌组织延迟发光强度的峰值分别为63±20计数/毫秒（n = 6）和48±12计数/毫秒（n = 14）。
癌症组织自发光子发射的强度大多与正常组织的强度有区别，并对它们的延迟发光特性进行了研究。
