Boura E, Liebl D, Spísek R, Fric J, Marek M, Stokrová J, Holán V, Forstová J
Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicná 5, 128 44 Prague 2, Czech Republic.
FEBS Lett. 2005 Dec 5;579(29):6549-58. doi: 10.1016/j.febslet.2005.10.062. Epub 2005 Nov 10.
A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terminal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.
构建了一种用于制备小鼠多瘤病毒衣壳样颗粒的载体，用于将外源肽或蛋白质转移到细胞中。已制备了携带与VP3次要蛋白C末端部分融合的EGFP的模型假衣壳（EGFP-VLPs），并分析了它们被小鼠细胞内化和加工以及在体外激活小鼠和人树突状细胞（DC）的能力。EGFP-VLPs能高效进入小鼠上皮细胞、成纤维细胞以及人和小鼠DC，并被溶酶体和蛋白酶体加工处理。令人惊讶的是，它们在体外并未诱导DC共刺激分子上调或成熟标志物表达；然而，它们确实诱导了白细胞介素12的分泌。
