Rosa Antonella, Deiana Monica, Corona Giulia, Atzeri Angela, Incani Alessandra, Appendino Giovanni, Dessì M Assunta
Dip. Biologia Sperimentale, Sez. Patologia Sperimentale, Università degli Studi di Cagliari, Cittadella Universitaria, Monserrato, Italy.
Free Radic Res. 2005 Nov;39(11):1155-62. doi: 10.1080/10715760500178094.
The activity of a single IP administration (15 or 30 mg/Kg body weight) of vanillyl nonanoate, a simplified analog of capsiate, on ferric nitrilotriacetate (Fe-NTA)-mediated oxidative damage was investigated. A sub-lethal dose of Fe-NTA (15 mg Fe/Kg body weight) was administered IP to rats; animals were sacrificed, and kidney and plasma were collected 1 h after injection. In response to the Fe-NTA administration, a reduction of the levels of total lipids, total unsaturated fatty acids and cholesterol was observed, accompanied by a rise in the concentrations of malondialdehyde (MDA), conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol in plasma and kidney 1 h after administration. A pre-treatment with synthetic capsiate (SCPT) showed remarkable protective effect on the reduction of the levels of total lipids, total unsaturated fatty acids and cholesterol, and the cellular antioxidant vitamin E, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol in the plasma and kidney. The protective effect of SCPT and two analogues (vanillyl alcohol and vanillin) during the linoleic acid and cholesterol oxidation was investigated in in vitro systems, providing evidence of definite structure-activity relationships.
研究了辣椒素类似物壬酸香草酯单次腹腔注射（15或30毫克/千克体重）对次氮基三乙酸铁（Fe-NTA）介导的氧化损伤的作用。将亚致死剂量的Fe-NTA（15毫克铁/千克体重）腹腔注射给大鼠；注射1小时后处死动物，收集肾脏和血浆。给予Fe-NTA后，观察到总脂质、总不饱和脂肪酸和胆固醇水平降低，同时给药1小时后血浆和肾脏中丙二醛（MDA）、共轭二烯脂肪酸氢过氧化物和7-酮胆固醇的浓度升高。用合成辣椒素（SCPT）预处理对总脂质、总不饱和脂肪酸和胆固醇水平以及细胞抗氧化剂维生素E的降低具有显著的保护作用，抑制了血浆和肾脏中MDA、共轭二烯脂肪酸氢过氧化物和7-酮胆固醇的增加。在体外系统中研究了SCPT及其两种类似物（香草醇和香草醛）在亚油酸和胆固醇氧化过程中的保护作用，提供了明确的构效关系证据。
