Yousefi-Mashouf R, Eley A, Duerden B I
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School.
J Med Microbiol. 1992 Aug;37(2):83-90. doi: 10.1099/00222615-37-2-83.
Gram-negative anaerobic bacilli previously known as the melaninogenicus-oralis group of Bacteroides have been assigned to a new genus, Prevotella. The non-pigmented members of this genus share several general characteristics and cannot be readily distinguished by routine tests. A polyacrylamide slab gel electrophoresis procedure, with visual analysis of protein patterns, was used to compare cellular protein patterns from clinical isolates with those from collection (reference) strains. Reference strains of P. oralis, P. veroralis, P. buccalis, P. oris, P. buccae, P. zoogleoformans, P. bivia, P. disiens, P. oulora, B. (P.) capillus and B. (P.) pentosaceus, and 91 non-pigmented isolates from patients with adult periodontal disease were examined by conventional biochemical tests, gas-liquid chromatography (GLC) and enzyme tests, and whole-cell protein profiles were obtained by SDS-PAGE. There was close correlation between patterns of results in biochemical and GLC tests and the SDS-PAGE profiles, and the species were readily distinguished in SDS-PAGE. The periodontal isolates were assigned to 10 groups by conventional test reaction patterns and nine groups by SDS-PAGE; the profiles of 79 isolates corresponded to those of seven species reference strains. By SDS-PAGE, clinical isolates of P. buccae (42 isolates) and P. oralis (eight isolates) showed good similarity with reference strains. However, for P. veroralis (15), P. oris (7), P. bivia (4), P. zoogleoformans (2) and P. buccalis (1), clinical isolates showed some minor variations from reference strains. Twelve isolates remained undesignated in SDS-PAGE analysis. Variant SDS-PAGE profiles divided clinical isolates of P. buccae into two subgroups and those of P. veroralis into five subgroups.
以前被称为拟杆菌属产黑色素口腔菌群的革兰氏阴性厌氧杆菌已被归入一个新属——普雷沃菌属。该属的无色素成员具有几个共同特征，通过常规试验难以轻易区分。采用一种聚丙烯酰胺平板凝胶电泳程序，并对蛋白质图谱进行视觉分析，以比较临床分离株与保藏（参考）菌株的细胞蛋白质图谱。通过常规生化试验、气液色谱法（GLC）和酶试验，对口腔普雷沃菌、口腔普氏菌、颊普雷沃菌、口普雷沃菌、颊普雷沃菌、菌胶团普雷沃菌、二路普雷沃菌、解脲普雷沃菌、卵形普雷沃菌、毛发普雷沃菌（拟杆菌属）和戊糖普雷沃菌（拟杆菌属）的参考菌株，以及来自成年牙周病患者的91株无色素分离株进行了检测，并通过SDS-PAGE获得了全细胞蛋白质谱。生化试验和GLC试验结果模式与SDS-PAGE谱之间存在密切相关性，并且在SDS-PAGE中很容易区分这些菌种。通过常规试验反应模式，牙周分离株被分为10组，通过SDS-PAGE分为9组；79株分离株的图谱与7个菌种参考菌株的图谱相对应。通过SDS-PAGE，颊普雷沃菌（42株分离株）和口腔普雷沃菌（8株分离株）的临床分离株与参考菌株显示出良好的相似性。然而，对于口腔普氏菌（15株）、口普雷沃菌（7株）、二路普雷沃菌（4株）、菌胶团普雷沃菌（2株）和颊普雷沃菌（1株），临床分离株与参考菌株显示出一些细微差异。在SDS-PAGE分析中有12株分离株未被鉴定。SDS-PAGE谱的变异将颊普雷沃菌的临床分离株分为两个亚组，将口腔普氏菌的临床分离株分为五个亚组。
