Duerden B I, Eley A, Goodwin L, Magee J T, Hindmarch J M, Bennett K W
Department of Medical Microbiology, University of Sheffield Medical School.
J Med Microbiol. 1989 May;29(1):63-73. doi: 10.1099/00222615-29-1-63.
Clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as Bacteroides ureolyticus) from superficial ulcers and soft tissue infections (15), non-gonococcal, non-chlamydial urethritis (12) and adult periodontal disease (14) were compared with reference strains of B. ureolyticus, B. gracilis and Wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatographic analysis of metabolic products, and in whole-cell analysis by pyrolysis-mass spectrometry (Py-MS). A numerical taxonomic approach was used with the results of conventional tests and the grouping obtained was compared with that obtained by Py-MS. All the ulcer and soft-tissue isolates and the urethritis isolates were oxidase- and urease-positive and formed a homogeneous set consistent with the reference strain of B. ureolyticus. The dental isolates differed from B. ureolyticus strains and were heterogeneous amongst themselves. None corresponded with the reference strains of B. gracilis or W. recta. The conventional and Py-MS approaches to characterisation produced similar groupings and each distinguished between a single cluster of ulcer-urethritis strains and several clusters of dental strains, although the dendrograms derived from the two approaches differed in the order of the clusters; in the Py-MS dendrogram one subcluster of four dental strains came within the main ulcer-urethritis cluster and a cluster of five ulcer strains was separated as a distinct group.
从浅表溃疡和软组织感染（15例）、非淋菌性非衣原体尿道炎（12例）及成人牙周病（14例）中分离出的具有腐蚀作用的革兰氏阴性厌氧杆菌（暂鉴定为解脲拟杆菌）临床分离株，与解脲拟杆菌、纤细拟杆菌和直肠沃氏菌的参考菌株进行了一系列常规检测比较，包括形态学、生化活性、对染料和胆盐的耐受性、抗生素敏感性、代谢产物的气液色谱分析以及热解质谱全细胞分析（Py-MS）。采用数值分类学方法对常规检测结果进行分析，并将所得分组与Py-MS分析结果进行比较。所有溃疡和软组织分离株以及尿道炎分离株均氧化酶和脲酶阳性，形成一组与解脲拟杆菌参考菌株一致的同源菌株。牙科分离株与解脲拟杆菌菌株不同，且彼此之间存在异质性。没有一个与纤细拟杆菌或直肠沃氏菌的参考菌株相符。传统方法和Py-MS方法对菌株特征的鉴定产生了相似的分组，并且每种方法都能区分出溃疡-尿道炎菌株的单个簇和几个牙科菌株簇，尽管两种方法得出的树状图中簇的顺序不同；在Py-MS树状图中，四个牙科菌株的一个亚簇位于主要的溃疡-尿道炎簇内，而五个溃疡菌株的一个簇被分离为一个独立的组。
