Moncla B J, Strockbine L, Braham P, Karlinsey J, Roberts M C
Department of Oral Biology, School of Dentistry, University of Washington, Seattle 98195.
J Dent Res. 1988 Oct;67(10):1267-70. doi: 10.1177/00220345880670100401.
Bacteroides intermedius includes two distinct groups of organisms that are phenotypically indistinguishable by conventional methods. These two groups are represented by the type strain of the species ATCC 25611T (B. intermedius type I) and by ATCC 33563 (B. intermedius type II). Members of each group can be distinguished from each other by analysis of the cellular protein composition by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by DNA-DNA homology studies, because they share less than 40% homology. The purpose of this study was to prepare specific DNA probes for the two groups of Bacteroides intermedius and to test them against field isolates. Whole-cell DNA probes were prepared from B. intermedius types I and II and tested against 253 field strains of Bacteroides which had been identified by conventional phenotypic tests as B. intermedius. Of these, 170 (67%) hybridized with the B. intermedius type I DNA probe, 28 (11%) with the type II, and 23 (9%) failed to react with the B. intermedius probes but did hybridize with either B. melaninogenicus, B. loescheii, or B. corporis whole-cell DNA probes. The 32 (13%) remaining isolates failed to hybridize with any of the five Bacteroides probes or with probes to B. asaccharolyticus, B. buccae, B. buccalis, B. denticola, B. gingivalis, B. oralis, or B. oris. These data demonstrate the usefulness of whole-cell DNA probes for the identification of phenotypically similar or identical field isolates.
中间普雷沃菌包括两组不同的微生物，用传统方法在表型上无法区分它们。这两组分别由该菌种的模式菌株ATCC 25611T（中间普雷沃菌I型）和ATCC 33563（中间普雷沃菌II型）代表。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析细胞蛋白质组成以及进行DNA-DNA同源性研究，可以区分每组中的成员，因为它们的同源性低于40%。本研究的目的是为两组中间普雷沃菌制备特异性DNA探针，并针对现场分离株进行检测。从中间普雷沃菌I型和II型制备全细胞DNA探针，并针对253株经传统表型试验鉴定为中间普雷沃菌的现场分离株进行检测。其中，170株（67%）与中间普雷沃菌I型DNA探针杂交，28株（11%）与II型杂交，23株（9%）与中间普雷沃菌探针不反应，但与产黑色素普雷沃菌、洛氏普雷沃菌或人体普雷沃菌的全细胞DNA探针杂交。其余32株（13%）分离株与五种普雷沃菌探针中的任何一种以及与解糖普雷沃菌、颊普雷沃菌、口腔普雷沃菌、齿龈普雷沃菌、牙龈普雷沃菌、口普雷沃菌或口腔普氏菌的探针均不杂交。这些数据证明了全细胞DNA探针在鉴定表型相似或相同的现场分离株方面的有用性。
