Shiea Jentaie, Huang Min-Zon, Hsu Hsiu-Jung, Lee Chi-Yang, Yuan Cheng-Hui, Beech Iwona, Sunner Jan
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424 Taiwan.
Rapid Commun Mass Spectrom. 2005;19(24):3701-4. doi: 10.1002/rcm.2243.
A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.
一种将激光解吸与电喷雾后电离相结合的新型电喷雾辅助激光解吸/电离（ELDI）质谱方法，被应用于环境条件下固体材料的快速分析。使用脉冲氮激光从固体金属和绝缘基质上解吸分析物。后电离产生了具有电喷雾特征的高质量质谱，包括蛋白质多重电荷。首次在不添加基质的情况下，通过激光解吸获得了完整蛋白质的质谱。本研究选择牛细胞色素c和一种含甲喹酮的违禁药物，以证明ELDI在蛋白质和合成有机化合物分析中的适用性。
