Tibesar Robert J, Moore Eric J, Bite Uldis
Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Arch Facial Plast Surg. 2005 Nov-Dec;7(6):398-404. doi: 10.1001/archfaci.7.6.398.
To assess the utility of distraction osteogenesis (DO) when applied to closure of a hard palate cleft in dogs.
A midline hard palate cleft was created in 10 mature dogs. Two were controls and had no distraction; the other 8 dogs underwent osteotomies with installation of customized DO devices to the hard palate. After a 10-day latency, distraction commenced at 1 mm/d. After a 14-day consolidation period, the device was removed and the mucosa closed. Each dog was injected with fluorochrome labels and serially killed at 2-week intervals. Bone healing was analyzed further with traditional histologic analysis and fluorochrome labeling.
No serious complications occurred. Bone resorption and cleft widening occurred in both control dogs. Complete bone closure of the hard palate cleft was achieved with DO in 5 of 8 experimental dogs. Three experimental dogs had bone resorption and incomplete palatal closure.
The application of DO techniques in closure of a hard palate cleft in a canine model is safe and well tolerated. Furthermore, in some cases, it proved effective in achieving bony closure of the cleft. Further investigation is warranted into innovative use of DO in treating children born with cleft palate.
评估牵张成骨术（DO）应用于犬硬腭裂闭合时的效用。
在10只成年犬中制造硬腭中线腭裂。2只为对照组，不进行牵张；其他8只犬接受截骨术，并在硬腭安装定制的DO装置。经过10天的延迟期后，以每天1毫米的速度开始牵张。经过14天的巩固期后，取出装置并闭合黏膜。每只犬注射荧光染料标记物，并每隔2周依次处死。采用传统组织学分析和荧光染料标记进一步分析骨愈合情况。
未发生严重并发症。两只对照犬均出现骨吸收和腭裂增宽。8只实验犬中有5只通过DO实现了硬腭裂的完全骨闭合。3只实验犬出现骨吸收和腭闭合不全。
DO技术应用于犬硬腭裂闭合模型是安全且耐受性良好的。此外，在某些情况下，它被证明在实现腭裂的骨性闭合方面是有效的。有必要进一步研究DO在治疗腭裂患儿中的创新应用。
