Rudney J D, Chen R, Zhang G
Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, 17-252 Moos Tower, 515 Delaware St. SE, Minneapolis, MN 55455, USA.
J Dent Res. 2005 Dec;84(12):1165-71. doi: 10.1177/154405910508401214.
Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green-labeled species-specific probes were accompanied by other bacteria recognized only by a red-labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species (G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.
此前，我们报道过，在人类受试者的颊上皮细胞内存在伴放线放线杆菌、牙龈卟啉单胞菌和福赛坦氏菌，它们作为多菌种菌群的次要成分存在。在本研究中，我们通过使用相同的双标记技术进一步对该细胞内菌群进行了表征，以鉴定颊上皮细胞内的具核梭杆菌、中间普氏菌、口腔弯曲菌属菌种、腐蚀埃肯菌、齿垢密螺旋体、溶血孪生球菌、毗邻颗粒链菌以及总链球菌。所有这些菌种均在颊细胞内被发现。在每种情况下，被绿色标记的种特异性探针识别的菌种都伴随着仅被红色标记的通用探针识别的其他细菌。链球菌似乎是多菌种细胞内菌群的主要成分，其存在水平比下一个最常见的菌种（毗邻颗粒链菌）高1至2个对数级。这与在口腔生物膜中观察到的情况类似，在口腔生物膜中，多种菌种在通常由链球菌主导的复杂群落中相互作用。
