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酵母双链RNA病毒L-A的外壳蛋白与真核生物mRNA的帽结构共价结合。

The coat protein of the yeast double-stranded RNA virus L-A attaches covalently to the cap structure of eukaryotic mRNA.

作者信息

Blanc A, Goyer C, Sonenberg N

机构信息

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

出版信息

Mol Cell Biol. 1992 Aug;12(8):3390-8. doi: 10.1128/mcb.12.8.3390-3398.1992.

DOI:10.1128/mcb.12.8.3390-3398.1992
PMID:1630453
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC364587/
Abstract

The eukaryotic mRNA 5' cap structure m7GpppX (where X is any nucleotide) interacts with a number of cellular proteins. Several of these proteins were studied in mammalian, yeast, and drosophila cells and found to be involved in translation initiation. Here we describe a novel cap-binding protein, the coat protein of L-A, a double-stranded RNA virus that is persistently maintained in many Saccharomyces cerevisiae strains. The results also suggest that the coat protein of a related double-stranded RNA virus (L-BC) is likewise a cap-binding protein. Strikingly, in contrast to the cellular cap-binding proteins, the interaction between the L-A virus coat protein and the cap structure is through a covalent bond.

摘要

真核生物信使核糖核酸（mRNA）的5'帽结构m7GpppX（其中X为任意核苷酸）与多种细胞蛋白相互作用。在哺乳动物、酵母和果蝇细胞中对其中几种蛋白进行了研究，发现它们参与翻译起始过程。在此，我们描述一种新型帽结合蛋白，即L-A双链RNA病毒的外壳蛋白，该病毒在许多酿酒酵母菌株中持续存在。结果还表明，相关双链RNA病毒（L-BC）的外壳蛋白同样是一种帽结合蛋白。引人注目的是，与细胞帽结合蛋白不同，L-A病毒外壳蛋白与帽结构之间的相互作用是通过共价键实现的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/59e73ca02214/molcellb00030-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/3d77312c510f/molcellb00030-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/bc196dc0313e/molcellb00030-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/7adce6d1b74e/molcellb00030-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/c8e4d4bcbdbc/molcellb00030-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/1260e46a5a29/molcellb00030-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/4c0e6c2e3edd/molcellb00030-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/5b13d7c01077/molcellb00030-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/296933197a3a/molcellb00030-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/59e73ca02214/molcellb00030-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/3d77312c510f/molcellb00030-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/bc196dc0313e/molcellb00030-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/7adce6d1b74e/molcellb00030-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/c8e4d4bcbdbc/molcellb00030-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/1260e46a5a29/molcellb00030-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/4c0e6c2e3edd/molcellb00030-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/5b13d7c01077/molcellb00030-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/296933197a3a/molcellb00030-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bca/364587/59e73ca02214/molcellb00030-0093-a.jpg

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Cap snatching of yeast L-A double-stranded RNA virus can operate in trans and requires viral polymerase actively engaging in transcription.酵母 L-A 双链 RNA 病毒的帽子抢夺可以在转译中进行，并且需要病毒聚合酶积极参与转录。
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