Gönczöl E, Váczi L, Boldogh I
Acta Microbiol Acad Sci Hung. 1975;22(1):19-26.
The virion synthesis by five human cytomegalovirus (CMV) strains in human embryonic fibroblast cultures was stopped by incubation of the infected cultures at 40 degrees C. At this temperature the antigens appeared diffusely filling the nucleus and the cytoplasm. The blocking effect of the elevated temperature was exerted in the same period of the reproduction cycle as the inhibitory effect of cytosine arabinoside (ara-C). In cell cultures infected with CMV and incubated first at 40 degrees C, then at 37 degrees C, the synthesis of infectious virus started again, thus the abortive cycle developed at 40 degrees C was reversible. The inhibition of virus multiplication cannot be attributed to the thermosensitive events in the normal function of the host cell.
通过将感染的人胚成纤维细胞培养物在40℃孵育，可阻止5株人巨细胞病毒（CMV）毒株在人胚成纤维细胞培养物中的病毒体合成。在此温度下，抗原呈弥漫状充满细胞核和细胞质。高温的阻断作用与阿糖胞苷（ara-C）的抑制作用在繁殖周期的同一时期发挥作用。在先用40℃孵育、再用37℃孵育的CMV感染细胞培养物中，传染性病毒的合成再次开始，因此在40℃形成的流产周期是可逆的。病毒增殖的抑制不能归因于宿主细胞正常功能中的热敏感事件。
