Gönczöl E, Boldogh I, Váczi L
Acta Microbiol Acad Sci Hung. 1975;22(3):263-70.
In arginine-deprived human embryonic fibroblasts the reproduction cycle of human cytomegalovirus (CMV) is incomplete. Infectious virus cannot be demonstrated in cell disintergrates, and from among the CMV antigens only the diffuse cytoplasmic antigen is detectable by immunofluorescence. The antigens localized in the cell membrane and those appearing during the complete cycle as large granules or inclusion-like bodies in the nucleus do not appear in the absence of arginine. The CMV genome persists in the arginine-deprived culture; after re-feeding with arginine-containing medium, maturation of virions soon ensues. Maturation could be prevented by inhibitors of protein synthesis, but not by DNA inhibitors, added simultaneously with completion of the medium.
在缺乏精氨酸的人胚胎成纤维细胞中，人巨细胞病毒（CMV）的复制周期是不完整的。在细胞裂解物中无法检测到感染性病毒，并且通过免疫荧光法，在CMV抗原中只能检测到弥漫性细胞质抗原。位于细胞膜上的抗原以及在完整周期中出现在细胞核中作为大颗粒或包涵体样结构的抗原，在缺乏精氨酸的情况下不会出现。CMV基因组在缺乏精氨酸的培养物中持续存在；在用含精氨酸的培养基重新培养后，病毒粒子很快就会成熟。蛋白质合成抑制剂可阻止成熟，但在培养基添加完成时同时添加的DNA抑制剂则不能。
