Kantor L T, Kominos S D, Yee R B
Am J Med Technol. 1975 Jan;41(1):3-9.
A simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. These organisms included apyocyanogenic Pseudomonas aeruginosa, P. fluorescens, P. putida, P. stutzeri, P. maltophilia, P. putrefaciens, P. cepacia, P. alcaligenes, FLAVOBACTERIUM SPECIES, Bordetella bronchiseptica, Acinetobacter anitratum (Herellea vaginicola), A. Iwoffi (Mima polymorpha), Moraxella species, Alcaligenes odorans and Alcaligenes species. The tests used for identification included production of cytochrome oxidase, amylase, deoxyribonuclease, gelatinase, urease and Beta-galactosidase; motility; oxidation of one per cent glucose and ten per cent lactose; fluorescence; indole, hydrogen sulfide and nitrogen gas production; denitrification of nitrites; growth at 42C; penicillin sensitivity and production of an aromatic odor and greenish discoloration on blood agar. Using this scheme, 85 per cent of 243 isolates (unknowns and reference strains) were identified to genus and species. Of the 15 per cent remaining, 11 per cent were identified as alkaline organisms and four per cent were unidentifiable.
已制定出一种简化、简洁的方案，用于鉴定文献中最常报道的作为人类疾病确诊或可能病原体的非发酵革兰氏阴性菌。这些微生物包括不产绿脓素的铜绿假单胞菌、荧光假单胞菌、恶臭假单胞菌、施氏假单胞菌、嗜麦芽窄食单胞菌、腐败假单胞菌、洋葱伯克霍尔德菌、产碱杆菌、黄杆菌属、支气管败血波氏杆菌、抗硝酸盐不动杆菌（阴道海雷氏菌）、沃氏不动杆菌（多形模仿菌）、莫拉克斯氏菌属、气味产碱杆菌和产碱杆菌属。用于鉴定的试验包括细胞色素氧化酶、淀粉酶、脱氧核糖核酸酶、明胶酶、脲酶和β-半乳糖苷酶的产生；运动性；1%葡萄糖和10%乳糖的氧化；荧光；吲哚、硫化氢和氮气的产生；亚硝酸盐的反硝化作用；42℃生长；青霉素敏感性以及在血琼脂上产生芳香气味和绿色变色。使用该方案，243株分离菌（未知菌和参考菌株）中有85%被鉴定到属和种。在其余15%中，11%被鉴定为嗜碱菌，4%无法鉴定。
