临床实验室中非发酵革兰氏阴性杆菌的鉴别
Differentiation of nonfermentative gram-negative bacilli in the clinical laboratory.
作者信息
Thong M L
出版信息
Southeast Asian J Trop Med Public Health. 1977 Mar;8(1):7-12.
A rapid and simplified system for the differentiation of nonfermentative Gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. Nonfermentative bacteria can be grouped initially by the motility, oxidase and OF glucose reactions. This grouping simplifies the choice of additional tests for further identification. The additional tests included Gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase activity, fluorescein production, deoxyribonuclease activity, hydrolysis of aesculin, growth at 42 degrees C, gelatinase activity and susceptibility to antibiotics.
本文介绍了一种快速简便的系统，用于区分临床标本中常见的非发酵革兰氏阴性杆菌，供临床实验室使用。非发酵菌最初可根据动力、氧化酶和OF葡萄糖反应进行分组。这种分组简化了用于进一步鉴定的其他试验的选择。其他试验包括革兰氏染色、10%乳糖琼脂产酸、硝酸盐还原、精氨酸双水解酶活性、荧光素产生、脱氧核糖核酸酶活性、七叶苷水解、42℃生长、明胶酶活性以及对抗生素的敏感性。
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